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<p>The current study aimed to research the bioactivity of Janus-like CuO–Ag nanoparticles (NPs) against clinical isolates of <i>Klebsiella pneumoniae</i>. The synthesis of CuO–Ag NPs was carried out by electric explosion of two twisted wires (EETW) method. The bioactivity of Janus-like CuO–Ag NPs was assessed by the determination of antibiofilm, antiadhesion, antibiotic sensitivity effect, and cytotoxicity on 3T3 cell line. The results indicate that the antibiofilm activity of these Janus-like CuO–Ag particles at subinhibitory concentrations was found to be between 2 and 10 times more potent than that of a mechanical mixture of CuO and Ag, separated Cu and Ag. Furthermore, the effective concentrations necessary for antiadhesion activity against<i> K</i>. <i>pneumoniae</i> were significantly lower for the Janus-like CuO–Ag particles, recorded at 2.0 and 10.0&#xa0;µg/ml, in contrast to the control, which showed values of 7.3 ± 0.2% and 46.7 ± 0.4%, respectively (<i>P</i> &lt; 0.05). Along with this, Janus-like CuO–Ag NPs increased the diameters of the antibiotic inhibition zones <i>K. pneumoniae</i>: for Imipenem from 5.0 ± 0.8 to 10.0 ± 1.0&#xa0;mm, Gentamicin from 15.0 ± 2.0 to 20 ± 2.0&#xa0;mm, and Amikacin from 13.0 ± 2.0 to 21 ± 2.0&#xa0;mm (<i>p</i> &lt; 0.05). We showed that ROS generation and Ag + release made only a small contribution to the bioactivity of CuO–Ag NPs. Notably, the generation of copper ions were increased from 7,5&#xa0;μg/L ± 0.1% to 25 ± 1% μg/L within 24&#xa0;h of exposure NPs (<i>P</i> &lt; 0.05). At the same time, the cytotoxicity test confirmed favorable safety profiles of the synthesized NPs.</p>

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The Bioactivity of Janus-like CuO–Ag Nanoparticles on Antibiotic-Resistant Clinical Isolates of Klebsiella pneumoniae and their Mechanism of Action

  • 期刊论文

  • 发表日期：

    2025年8月4日

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  • Marina V. Fomina,
  • Irina V. Gladysheva,
  • Olga V. Bakina,
  • Sergey V. Cherkasov,
  • Ilshat F. Karimov

摘要

The current study aimed to research the bioactivity of Janus-like CuO–Ag nanoparticles (NPs) against clinical isolates of Klebsiella pneumoniae. The synthesis of CuO–Ag NPs was carried out by electric explosion of two twisted wires (EETW) method. The bioactivity of Janus-like CuO–Ag NPs was assessed by the determination of antibiofilm, antiadhesion, antibiotic sensitivity effect, and cytotoxicity on 3T3 cell line. The results indicate that the antibiofilm activity of these Janus-like CuO–Ag particles at subinhibitory concentrations was found to be between 2 and 10 times more potent than that of a mechanical mixture of CuO and Ag, separated Cu and Ag. Furthermore, the effective concentrations necessary for antiadhesion activity against K. pneumoniae were significantly lower for the Janus-like CuO–Ag particles, recorded at 2.0 and 10.0 µg/ml, in contrast to the control, which showed values of 7.3 ± 0.2% and 46.7 ± 0.4%, respectively (P < 0.05). Along with this, Janus-like CuO–Ag NPs increased the diameters of the antibiotic inhibition zones K. pneumoniae: for Imipenem from 5.0 ± 0.8 to 10.0 ± 1.0 mm, Gentamicin from 15.0 ± 2.0 to 20 ± 2.0 mm, and Amikacin from 13.0 ± 2.0 to 21 ± 2.0 mm (p < 0.05). We showed that ROS generation and Ag + release made only a small contribution to the bioactivity of CuO–Ag NPs. Notably, the generation of copper ions were increased from 7,5 μg/L ± 0.1% to 25 ± 1% μg/L within 24 h of exposure NPs (P < 0.05). At the same time, the cytotoxicity test confirmed favorable safety profiles of the synthesized NPs.