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<p>Antibiotics, a milestone invention in modern medicine, have significantly improved human quality of life and serve as a primary weapon against bacterial infections. However, antibiotic abuse has led to the emergence and worsening of bacterial resistance, now a major global public health challenge. Therefore, the efficient use of nanoscale antimicrobial peptides (APs) as therapeutic and diagnostic agents offers a novel strategy to replace conventional antibiotics. In this study, we innovatively combined AP with manganese dioxide (MnO<sub>2</sub>) and zeolitic imidazolate framework-8 (ZIF-8) to develop a novel composite: ZIF-8-encapsulated and AP-loaded MnO<sub>2</sub> (AP-MnO<sub>2</sub>@ZIF-8). This composite exhibited superior properties, including enhanced biocompatibility, excellent aqueous stability, and increased antibacterial activity. Both in vitro and in vivo AP-MnO<sub>2</sub>@ZIF-8 demonstrated potent efficacy against multidrug-resistant (MDR) <i>Escherichia coli</i> (<i>E. coli</i>) and <i>Staphylococcus aureus</i> (<i>S. aureus</i>). Its minimum inhibitory concentrations (MICs) were 20&#xa0;μg/ml for <i>E. coli</i> and 39&#xa0;μg/ml for <i>S. aureus</i>. Furthermore, colony-forming unit (CFU) assays showed that 150-μg/ml AP-MnO<sub>2</sub>@ZIF-8 reduced bacterial survival below 5%, indicating effective growth inhibition. Crucially, it significantly suppressed bacterial activity in <i>S. aureus</i>-infected mouse wound models. Compared to free AP or MnO<sub>2</sub> alone, AP-MnO<sub>2</sub>@ZIF-8 showed significantly enhanced water solubility, bioavailability, and antibacterial activity. This work provides a foundation for developing AP-based antimicrobial strategies and offers a novel nanomaterial approach to combat the increasingly severe antibiotic resistance crisis. </p> Graphical Abstract <p></p>

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Synthesis of ZIF-8 Coated MnO2 Functionalized Antibacterial Peptide Nanoparticles and Its Antibacterial and Wound-Healing Promotion Studies

  • 期刊论文

  • 发表日期：

    2025年7月26日

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  • Mengzhu Xiao,
  • Yujia Wu,
  • Wanzhen Li,
  • Jun Wang,
  • Weiwei Zhang,
  • Lin Gui,
  • Fei Ge

摘要

Antibiotics, a milestone invention in modern medicine, have significantly improved human quality of life and serve as a primary weapon against bacterial infections. However, antibiotic abuse has led to the emergence and worsening of bacterial resistance, now a major global public health challenge. Therefore, the efficient use of nanoscale antimicrobial peptides (APs) as therapeutic and diagnostic agents offers a novel strategy to replace conventional antibiotics. In this study, we innovatively combined AP with manganese dioxide (MnO2) and zeolitic imidazolate framework-8 (ZIF-8) to develop a novel composite: ZIF-8-encapsulated and AP-loaded MnO2 (AP-MnO2@ZIF-8). This composite exhibited superior properties, including enhanced biocompatibility, excellent aqueous stability, and increased antibacterial activity. Both in vitro and in vivo AP-MnO2@ZIF-8 demonstrated potent efficacy against multidrug-resistant (MDR) Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus). Its minimum inhibitory concentrations (MICs) were 20 μg/ml for E. coli and 39 μg/ml for S. aureus. Furthermore, colony-forming unit (CFU) assays showed that 150-μg/ml AP-MnO2@ZIF-8 reduced bacterial survival below 5%, indicating effective growth inhibition. Crucially, it significantly suppressed bacterial activity in S. aureus-infected mouse wound models. Compared to free AP or MnO2 alone, AP-MnO2@ZIF-8 showed significantly enhanced water solubility, bioavailability, and antibacterial activity. This work provides a foundation for developing AP-based antimicrobial strategies and offers a novel nanomaterial approach to combat the increasingly severe antibiotic resistance crisis.

Graphical Abstract