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<p>Phenotypic, physiological, phylogenetic, genomic, and chemotaxonomic analyses were carried out on a bacterium, designated as KMU-145<sup>T</sup>, isolated from seawater gathered in the Republic of Korea. Strain KMU-145<sup>T</sup> was ovoid-shaped, Gram-reaction-negative, non-motile, obligately aerobic, and formed apricot-colored colonies on marine agar 2216. The strain grew at 15–37&#xa0;°C, pH 6.0–9.5, and 0–2.0% NaCl concentrations (w/v). Preliminary evaluation based on 16S rRNA gene sequence showed that strain KMU-145<sup>T</sup> was affiliated with the genus <i>Jannaschia</i>, family <i>Roseobacteraceae</i>, showing the greatest sequence similarity (98.4%) to “<i>J. ovalis</i>” GRR-S6-38<sup>T</sup>. The only respiratory quinone was ubiquinone-10, and the main (&gt; 10%) fatty acids were C<sub>18:1</sub> <i>ω</i>7<i>c</i>, C<sub>18:1</sub> <i>ω</i>7<i>c</i> 11-methyl, and C<sub>18:0</sub>. Phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, three unidentified aminolipids, and three unidentified lipids were found as polar lipids. Strain KMU-145<sup>T</sup> had a 3.5 Mbp genome with a 68.5% G+C content. The pairwise ANI values between the genomes of strain KMU-145<sup>T</sup> and other species of the genus <i>Jannaschia</i> were 70.9–79.3%. Strain KMU-145<sup>T</sup> exhibited 21.6% <i>d</i>DDH value with strain “<i>J. ovalis</i>” GRR-S6-38<sup>T</sup>. The genome of strain KMU-145<sup>T</sup> contained numerous carbohydrate-active enzymes, suggesting a significant potential for the development of enzymes with practical applications in industry. Therefore, the marine strain represents a novel member of the genus <i>Jannaschia</i>, for which the name <i>Jannaschia halovivens</i> sp. nov., is proposed with the type strain KMU-145<sup>T</sup> (= KCCM 90597<sup>T</sup> = NBRC 117092<sup>T</sup>).</p>

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Characterization of Jannaschia halovivens sp. nov., Isolated from Seawater

  • 期刊论文

  • 发表日期：

    2025年7月2日

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  • Jaewoo Yoon,
  • Ji Hoon Oh

摘要

Phenotypic, physiological, phylogenetic, genomic, and chemotaxonomic analyses were carried out on a bacterium, designated as KMU-145T, isolated from seawater gathered in the Republic of Korea. Strain KMU-145T was ovoid-shaped, Gram-reaction-negative, non-motile, obligately aerobic, and formed apricot-colored colonies on marine agar 2216. The strain grew at 15–37 °C, pH 6.0–9.5, and 0–2.0% NaCl concentrations (w/v). Preliminary evaluation based on 16S rRNA gene sequence showed that strain KMU-145T was affiliated with the genus Jannaschia, family Roseobacteraceae, showing the greatest sequence similarity (98.4%) to “J. ovalis” GRR-S6-38T. The only respiratory quinone was ubiquinone-10, and the main (> 10%) fatty acids were C18:1 ω7c, C18:1 ω7c 11-methyl, and C18:0. Phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, three unidentified aminolipids, and three unidentified lipids were found as polar lipids. Strain KMU-145T had a 3.5 Mbp genome with a 68.5% G+C content. The pairwise ANI values between the genomes of strain KMU-145T and other species of the genus Jannaschia were 70.9–79.3%. Strain KMU-145T exhibited 21.6% dDDH value with strain “J. ovalis” GRR-S6-38T. The genome of strain KMU-145T contained numerous carbohydrate-active enzymes, suggesting a significant potential for the development of enzymes with practical applications in industry. Therefore, the marine strain represents a novel member of the genus Jannaschia, for which the name Jannaschia halovivens sp. nov., is proposed with the type strain KMU-145T (= KCCM 90597T = NBRC 117092T).