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<p>A Gram-stain-negative, rod-shaped, strictly aerobic, non-motile, and chemo-organoheterotrophic alphaproteobacterium, designated KMU-169<sup>T</sup>, was isolated from coastal seawater in the Republic of Korea. The novel isolate was able to grow at 0–2.0% NaCl concentrations (w/v), pH 6.0–9.5, and 15–40&#xa0;°C. The analysis based on 16S rRNA gene sequences indicated that strain KMU-169<sup>T</sup> belongs to the family <i>Paracoccaceae</i> and shared the highest similarity (97.0%) with “<i>Defluviimonas salinarum</i>” CAU 1641<sup>T</sup>. The major (&gt;&#xa0;10%) cellular fatty acids were C<sub>18:1</sub> <i>ω</i>7<i>c</i> and summed feature 3 (comprising C<sub>16:1</sub> <i>ω</i>6<i>c</i> and/or C<sub>16:1</sub> <i>ω</i>7<i>c</i>). The predominant respiratory quinone was ubiquinone-10. Strain KMU-169<sup>T</sup> comprised phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine, an unidentified aminolipid, an unidentified phospholipid, and an unidentified lipid. The assembled draft genome size of strain KMU-169<sup>T</sup> was 4.1 Mbp with a DNA G + C content of 65.0%. The average nucleotide identity (ANI), average amino acid identity (AAI), and digital DNA–DNA hybridization (<i>d</i>DDH) values between the genomes of strain KMU-169<sup>T</sup> and its closely related taxa were 71.9–76.8%, 60.9–76.1%, and 18.4–21.0%, respectively. The genome of the strain KMU-169<sup>T</sup> revealed the presence of numerous genes involved in bio-macromolecule degradation, indicating a high potential for producing industrially valuable enzymes. Based on the polyphasic taxonomic data reported in this study, a novel genus and a new species of the family <i>Paracoccaceae</i>, for which the name <i>Pontitalea aquivivens</i> gen. nov., sp. nov., is proposed with the type strain KMU-169<sup>T</sup> (=&#xa0;KCCM 90598<sup>T</sup> = NBRC 117093<sup>T</sup>).</p>

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Polyphasic Taxonomic Analysis of Pontitalea aquivivens gen. nov., sp. nov., Isolated from Seawater

  • 期刊论文

  • 发表日期：

    2025年5月27日

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  • Jaewoo Yoon

摘要

A Gram-stain-negative, rod-shaped, strictly aerobic, non-motile, and chemo-organoheterotrophic alphaproteobacterium, designated KMU-169T, was isolated from coastal seawater in the Republic of Korea. The novel isolate was able to grow at 0–2.0% NaCl concentrations (w/v), pH 6.0–9.5, and 15–40 °C. The analysis based on 16S rRNA gene sequences indicated that strain KMU-169T belongs to the family Paracoccaceae and shared the highest similarity (97.0%) with “Defluviimonas salinarum” CAU 1641T. The major (> 10%) cellular fatty acids were C18:1 ω7c and summed feature 3 (comprising C16:1 ω6c and/or C16:1 ω7c). The predominant respiratory quinone was ubiquinone-10. Strain KMU-169T comprised phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine, an unidentified aminolipid, an unidentified phospholipid, and an unidentified lipid. The assembled draft genome size of strain KMU-169T was 4.1 Mbp with a DNA G + C content of 65.0%. The average nucleotide identity (ANI), average amino acid identity (AAI), and digital DNA–DNA hybridization (dDDH) values between the genomes of strain KMU-169T and its closely related taxa were 71.9–76.8%, 60.9–76.1%, and 18.4–21.0%, respectively. The genome of the strain KMU-169T revealed the presence of numerous genes involved in bio-macromolecule degradation, indicating a high potential for producing industrially valuable enzymes. Based on the polyphasic taxonomic data reported in this study, a novel genus and a new species of the family Paracoccaceae, for which the name Pontitalea aquivivens gen. nov., sp. nov., is proposed with the type strain KMU-169T (= KCCM 90598T = NBRC 117093T).