Scholarlink

<p>Antimicrobial resistance (AMR) imperils global public health overlooking the epidemiological situation of community-acquired infections, especially in low- and middle-income countries. We conducted this study in a suburban community in West Bengal, India, to comprehend the AMR and bacterial forensics of <i>Escherichia coli</i> causing community-acquired urinary tract infections (CAUTIs). Antibiograms, β-lactamases, plasmid-mediated quinolone resistance genes, and plasmids were investigated to explore AMR. Bacterial forensics was assessed using phylogrouping, genetic fingerprinting, and strain typing. Antibiograms revealed that &gt; 75% of isolates had a multiple-antibiotic resistance (MAR) index of &gt; 0.2. Fosfomycin remained 100% effective as an empiric drug, followed by nitrofurantoin (&gt; 75%), aminoglycosides (&gt; 65%), and co-trimoxazole (&gt; 50%). Multidrug-resistant (MDR) was frequent (&gt; 75%), with &lt; 5% being extensively drug-resistant, including two colistin-resistant isolates. Piperacillin/tazobactam showed promising activity against the ESBL-producing isolates (&gt; 70%) mostly carrying CTX-M-15 and TEM-1 (&gt; 50%), which declined in the presence of OXA-1 (&gt; 20%). TEM-253 and 254, two novel alleles, were assigned. Two of the thirteen NDM-5-producing carbapenem-resistant isolates co-harbored OXA-48. Among fluoroquinolone-resistant isolates (&gt; 80%), <i>aac(6')-lb-cr</i> (&gt; 20%) was the&#xa0;most common, followed by <i>qnrS</i> (&gt; 10%). Plasmid of &gt; 212&#xa0;kb was prevalent (&gt; 60%), especially in MDR isolates. IncF was the sole plasmid in &gt; 85% of isolates, with &gt; 90% having a MAR index &gt; 0.2. Phylogroup B2 was prevalent (&gt; 35%), primarily associated with the ESBL-producing ST131 clone (&gt; 80%). Carbapenem-resistant isolates belonged to phylogroup A/sequence-type361, C/ST2851, D/ST405, and F/ST648. ESBL-producing isolates were largely clonally related. This maiden molecular study reveals the infiltration of MDR epidemic clones in a suburban community in eastern India, guiding genomic surveillance for strategic management of AMR at the national level. </p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Molecular Characterization of Escherichia coli Causing Community-Acquired Urinary Tract Infections in a Suburban Area in West Bengal, India: Dispersal of Multidrug-Resistant Epidemic ST131 Clone Carrying CTX-M-1

  • 期刊论文

  • 发表日期：

    2025年5月23日

查看全文
  • Urmy Biswas,
  • Abhi Mallick,
  • Taru Singh,
  • Subhadip Das,
  • Surojit Das

摘要

Antimicrobial resistance (AMR) imperils global public health overlooking the epidemiological situation of community-acquired infections, especially in low- and middle-income countries. We conducted this study in a suburban community in West Bengal, India, to comprehend the AMR and bacterial forensics of Escherichia coli causing community-acquired urinary tract infections (CAUTIs). Antibiograms, β-lactamases, plasmid-mediated quinolone resistance genes, and plasmids were investigated to explore AMR. Bacterial forensics was assessed using phylogrouping, genetic fingerprinting, and strain typing. Antibiograms revealed that > 75% of isolates had a multiple-antibiotic resistance (MAR) index of > 0.2. Fosfomycin remained 100% effective as an empiric drug, followed by nitrofurantoin (> 75%), aminoglycosides (> 65%), and co-trimoxazole (> 50%). Multidrug-resistant (MDR) was frequent (> 75%), with < 5% being extensively drug-resistant, including two colistin-resistant isolates. Piperacillin/tazobactam showed promising activity against the ESBL-producing isolates (> 70%) mostly carrying CTX-M-15 and TEM-1 (> 50%), which declined in the presence of OXA-1 (> 20%). TEM-253 and 254, two novel alleles, were assigned. Two of the thirteen NDM-5-producing carbapenem-resistant isolates co-harbored OXA-48. Among fluoroquinolone-resistant isolates (> 80%), aac(6')-lb-cr (> 20%) was the most common, followed by qnrS (> 10%). Plasmid of > 212 kb was prevalent (> 60%), especially in MDR isolates. IncF was the sole plasmid in > 85% of isolates, with > 90% having a MAR index > 0.2. Phylogroup B2 was prevalent (> 35%), primarily associated with the ESBL-producing ST131 clone (> 80%). Carbapenem-resistant isolates belonged to phylogroup A/sequence-type361, C/ST2851, D/ST405, and F/ST648. ESBL-producing isolates were largely clonally related. This maiden molecular study reveals the infiltration of MDR epidemic clones in a suburban community in eastern India, guiding genomic surveillance for strategic management of AMR at the national level.