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<p>A Gram-negative, aerobic, rod-shaped, and non-motile bacterium, designated strain R1<sup>T</sup>, was isolated from lakeside soil in Nanchang, Jiangxi Province, China. Growth was observed at 15–37&#xa0;°C (optimum 30&#xa0;°C), pH 6–8 (optimum pH 7) and salinity of 0–1.0% (w/v; optimum 0.5%). Strain R1<sup>T</sup> completely degraded 5&#xa0;mg/L of ochratoxin A within 48&#xa0;h of incubation. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain R1<sup>T</sup> belongs to the genus <i>Terrimonas</i>, and showed 99% sequences similarity with <i>Terrimonas pollutisoli</i> H1YJ31<sup>T</sup>. The draft genome of strain R1<sup>T</sup> was approximately 6.2&#xa0;Mb with a 41.5% G + C content. The average nucleotide identity and digital DNA–DNA hybridization values between strain R1<sup>T</sup> and <i>T. pollutisoli</i> H1YJ31<sup>T</sup> were 90.4% and 41.2%, respectively. The predominant fatty acids of strain R1<sup>T</sup> were iso-C<sub>15:0</sub>, summed feature 3 (C<sub>16:1</sub><i> ω</i>6<i>c</i> and/or C<sub>16:1</sub><i> ω</i>7<i>c</i>), iso-C<sub>17:0</sub> 3-OH and iso-C<sub>15:1</sub> G. The major isoprenoid quinone was menaquinone 7 (MK-7). The major polar lipids of strain R1<sup>T</sup> were phosphatidylethanolamine, phosphatidylglycerol, a phospholipid, two unidentified glycolipids, two unidentified aminophospholipids, and five unidentified polar lipids. Based on phenotypic, genotypic and phylogenetic evidence, strain R1<sup>T</sup> represents a novel species in the genus <i>Terrimonas</i>, for which the name <i>Terrimonas alba</i> sp. nov. is proposed. The type strain is R1<sup>T</sup> (= GDMCC 1.4673<sup>&#xa0;T</sup> = JCM 37058<sup>&#xa0;T</sup> = KCTC 102293<sup>&#xa0;T</sup> = KACC 23743<sup>&#xa0;T</sup>). </p>

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Terrimonas alba sp. nov., an Ochratoxin A-Degrading Strain Isolated from Lakeside Soil

  • 期刊论文

  • 发表日期：

    2025年4月30日

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  • Miaohuan Xiong,
  • Huipeng Wu,
  • Jialu Li,
  • Yingying Wan,
  • Xuelan Chen,
  • Bin Liu,
  • Jian He

摘要

A Gram-negative, aerobic, rod-shaped, and non-motile bacterium, designated strain R1T, was isolated from lakeside soil in Nanchang, Jiangxi Province, China. Growth was observed at 15–37 °C (optimum 30 °C), pH 6–8 (optimum pH 7) and salinity of 0–1.0% (w/v; optimum 0.5%). Strain R1T completely degraded 5 mg/L of ochratoxin A within 48 h of incubation. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain R1T belongs to the genus Terrimonas, and showed 99% sequences similarity with Terrimonas pollutisoli H1YJ31T. The draft genome of strain R1T was approximately 6.2 Mb with a 41.5% G + C content. The average nucleotide identity and digital DNA–DNA hybridization values between strain R1T and T. pollutisoli H1YJ31T were 90.4% and 41.2%, respectively. The predominant fatty acids of strain R1T were iso-C15:0, summed feature 3 (C16:1 ω6c and/or C16:1 ω7c), iso-C17:0 3-OH and iso-C15:1 G. The major isoprenoid quinone was menaquinone 7 (MK-7). The major polar lipids of strain R1T were phosphatidylethanolamine, phosphatidylglycerol, a phospholipid, two unidentified glycolipids, two unidentified aminophospholipids, and five unidentified polar lipids. Based on phenotypic, genotypic and phylogenetic evidence, strain R1T represents a novel species in the genus Terrimonas, for which the name Terrimonas alba sp. nov. is proposed. The type strain is R1T (= GDMCC 1.4673 T = JCM 37058 T = KCTC 102293 T = KACC 23743 T).