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<p>A novel bacterium, designated STR12<sup>T</sup>, was isolated from the soil of a moso bamboo (<i>Phyllostachys edulis</i>) forest in Hangzhou, China. The strain was a Gram-stain-negative, rod-shaped, motile bacterium; the colonies of which were yellow, round, flat, sticky, and non-moist with a smooth margin after cultivation for 3&#xa0;days at 28&#xa0;°C. The strain grew at temperatures between 15 and 37&#xa0;°C (optimum, 28&#xa0;°C), pH values from 4.0 to 8.0 (optimum, pH 7.0), and salinities ranging from 0 to 5% (w/v) NaCl (optimum, 1%). Phylogenetic analyses based on the 16S rRNA gene sequence revealed that the strain was grouped within a cluster of the genus <i>Frateuria</i>, showing the highest similarity with <i>Frateuria flava</i> MAH-13<sup>T</sup> (97.7%). The genome of strain STR12<sup>T</sup> was 3.74&#xa0;Mb in length with a G+C content of 67.9%. Genome comparisons of strain STR12<sup>T</sup> with other species within the genus <i>Frateuria</i> revealed that the range of average nucleotide identity, DNA–DNA hybridization, and average amino acid identity values were 73.9–85.9%, 20.8–30.7%, and 62.7–86.7%, respectively, all of those were below the respective prokaryotic species delineation thresholds. The predominant quinone in strain STR12<sup>T</sup> was ubiquinone-8 and the major fatty acids were iso-C<sub>15:0</sub>, iso-C<sub>16:0</sub>, iso-C<sub>17:0</sub>, and Summed Feature 9. Polar lipids included diphosphatidylglycerol, phosphatidylethanolamine, two unidentified aminophospholipids, and one unidentified phosphoglycolipid. On basis of the finding of phylogenetic, physiological, and chemotaxonomic analyses, we proposed the name <i>Frateuria hangzhouensis</i> sp. nov. for the novel species in the genus <i>Frateuria</i>, of which the type strain was strain STR12<sup>T</sup> (= ACCC 61897<sup>T</sup> = GDMCC 1.2964<sup>T</sup> = JCM 35226<sup>T</sup>).</p>

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Frateuria hangzhouensis sp. nov. Isolated from Soil of Moso Bamboo Forest in Hangzhou, China

  • 期刊论文

  • 发表日期：

    2025年4月28日

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  • Fu Yang,
  • Jinjun Yue,
  • Qin Wang,
  • Weiwei Zha,
  • Yanxu Zhou,
  • Jinling Yuan,
  • Lubin Li,
  • Qiwu Sun,
  • Lei Liu

摘要

A novel bacterium, designated STR12T, was isolated from the soil of a moso bamboo (Phyllostachys edulis) forest in Hangzhou, China. The strain was a Gram-stain-negative, rod-shaped, motile bacterium; the colonies of which were yellow, round, flat, sticky, and non-moist with a smooth margin after cultivation for 3 days at 28 °C. The strain grew at temperatures between 15 and 37 °C (optimum, 28 °C), pH values from 4.0 to 8.0 (optimum, pH 7.0), and salinities ranging from 0 to 5% (w/v) NaCl (optimum, 1%). Phylogenetic analyses based on the 16S rRNA gene sequence revealed that the strain was grouped within a cluster of the genus Frateuria, showing the highest similarity with Frateuria flava MAH-13T (97.7%). The genome of strain STR12T was 3.74 Mb in length with a G+C content of 67.9%. Genome comparisons of strain STR12T with other species within the genus Frateuria revealed that the range of average nucleotide identity, DNA–DNA hybridization, and average amino acid identity values were 73.9–85.9%, 20.8–30.7%, and 62.7–86.7%, respectively, all of those were below the respective prokaryotic species delineation thresholds. The predominant quinone in strain STR12T was ubiquinone-8 and the major fatty acids were iso-C15:0, iso-C16:0, iso-C17:0, and Summed Feature 9. Polar lipids included diphosphatidylglycerol, phosphatidylethanolamine, two unidentified aminophospholipids, and one unidentified phosphoglycolipid. On basis of the finding of phylogenetic, physiological, and chemotaxonomic analyses, we proposed the name Frateuria hangzhouensis sp. nov. for the novel species in the genus Frateuria, of which the type strain was strain STR12T (= ACCC 61897T = GDMCC 1.2964T = JCM 35226T).