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<p>The biofilm-mediated persistence of <i>Pseudomonas aeruginosa</i> in the food and biomedical sectors is currently a global concern. In light of this challenge, this study investigated a preventive approach against <i>P. aeruginosa</i> biofilm formation using Flavourzyme, a food-grade peptidase, considering its antibiofilm potential. The results revealed that a co-culture comprising 300 µL/mL (1 × minimum inhibitory concentration [MIC]) of Flavourzyme could kill <i>P. aeruginosa</i>. On the MBEC™ biofilm-forming device, 0.125 × MIC of Flavourzyme inhibited &gt; 4.5 log CFU/peg of biofilm. Cell motilities and the biosynthesis of quorum sensing (QS) molecules such as N-acyl-homoserine lactones (AHLs), including C<sub>4</sub>-HSL, decreased significantly at 0.06 × MIC of Flavourzyme and became undetectable at 0.125 × MIC. Interestingly, while 0.03 × MIC of Flavourzyme elicited diverse expressions of QS and virulence-regulating genes, ≥ 0.06 × MIC of Flavourzyme remarkably suppressed the relative genomic expressions. This study proposes Flavourzyme as a potent antibiofilm agent against <i>P. aeruginosa</i> biofilms, recommending specific concentrations for effective use in food preservation.</p> Graphical Abstract <p></p>

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Flavourzyme Suppresses Pseudomonas aeruginosa Biofilms by Targeting Motility, Quorum Sensing, and Virulence Genes

  • 期刊论文

  • 发表日期：

    2025年4月10日

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  • Shamsun Nahar,
  • A. G. M. Sofi Uddin Mahamud,
  • Ah Jin Cho,
  • Md. Ashrafudoulla,
  • Jisu Yu,
  • Si Hong Park,
  • Sang-Do Ha

摘要

The biofilm-mediated persistence of Pseudomonas aeruginosa in the food and biomedical sectors is currently a global concern. In light of this challenge, this study investigated a preventive approach against P. aeruginosa biofilm formation using Flavourzyme, a food-grade peptidase, considering its antibiofilm potential. The results revealed that a co-culture comprising 300 µL/mL (1 × minimum inhibitory concentration [MIC]) of Flavourzyme could kill P. aeruginosa. On the MBEC™ biofilm-forming device, 0.125 × MIC of Flavourzyme inhibited > 4.5 log CFU/peg of biofilm. Cell motilities and the biosynthesis of quorum sensing (QS) molecules such as N-acyl-homoserine lactones (AHLs), including C4-HSL, decreased significantly at 0.06 × MIC of Flavourzyme and became undetectable at 0.125 × MIC. Interestingly, while 0.03 × MIC of Flavourzyme elicited diverse expressions of QS and virulence-regulating genes, ≥ 0.06 × MIC of Flavourzyme remarkably suppressed the relative genomic expressions. This study proposes Flavourzyme as a potent antibiofilm agent against P. aeruginosa biofilms, recommending specific concentrations for effective use in food preservation.

Graphical Abstract