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<p>Shiga toxin-producing <i>Escherichia coli</i> (STEC) is a zoonotic foodborne pathogen responsible for different cases and outbreaks of severe enteric diseases in humans worldwide. Our group previously showed that Argentine O22:H8 isolates (155 and 182) interfere with the colonization of O157:H7 in an experimental infection bovine model. In this study, we applied a comparative genomic and genome-wide association study (GWAS) analysis to explore the differences between the genetic bases of O22:H8 and O157:H7 strains isolated from cattle. The whole-genome sequence of O22:H8 155 and 182 isolates was compared with 33 O22:H8 and 156 O157:H7 isolates from bovine. Our results showed that O22:H8 isolates harbor genes associated with specific metabolic pathways related to the metabolism of products of vegetal origin, which are absent in O157:H7 isolates. These O22:H8 isolates also carry classical genes of the locus of adhesion and autoaggregation related to the adhesion of non-O157 isolates. In addition, we showed that the T6SS-1 cluster related to bacterial competition predicted in the Argentine O22:H8 isolates is absent both in O157:H7 and other O22:H8 isolates. We also predicted for the first time the locus of type VI secretion system 1 (LT6SS-T1), a genomic island that harbors both T6SS-1 cluster genes and genes related to STEC pathogenesis. This study reveals a subset of genes present in the Argentine O22:H8 isolates that could confer an advantage over O157:H7 during the colonization process in the bovine intestinal tract.</p>

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Whole-Genome Sequence Reveals Genetic Determinants of the Colonization of Shiga Toxin-Producing Escherichia coli O22:H8 Isolates in Cattle

  • 期刊论文

  • 发表日期：

    2025年3月25日

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  • Wanderson Marques Da Silva,
  • Libia Yael Smith,
  • Flavia Figueira Aburjaile,
  • Mariano Larzabal,
  • Sandy Ingrid Aguiar Alves,
  • Pablo Farace,
  • Carlos Willian Dantas,
  • Mateus Matiuzzi Costa,
  • Bertram Brenig,
  • Rommel Thiago Jucá Ramos,
  • Vasco Azevedo,
  • Angel Cataldi

摘要

Shiga toxin-producing Escherichia coli (STEC) is a zoonotic foodborne pathogen responsible for different cases and outbreaks of severe enteric diseases in humans worldwide. Our group previously showed that Argentine O22:H8 isolates (155 and 182) interfere with the colonization of O157:H7 in an experimental infection bovine model. In this study, we applied a comparative genomic and genome-wide association study (GWAS) analysis to explore the differences between the genetic bases of O22:H8 and O157:H7 strains isolated from cattle. The whole-genome sequence of O22:H8 155 and 182 isolates was compared with 33 O22:H8 and 156 O157:H7 isolates from bovine. Our results showed that O22:H8 isolates harbor genes associated with specific metabolic pathways related to the metabolism of products of vegetal origin, which are absent in O157:H7 isolates. These O22:H8 isolates also carry classical genes of the locus of adhesion and autoaggregation related to the adhesion of non-O157 isolates. In addition, we showed that the T6SS-1 cluster related to bacterial competition predicted in the Argentine O22:H8 isolates is absent both in O157:H7 and other O22:H8 isolates. We also predicted for the first time the locus of type VI secretion system 1 (LT6SS-T1), a genomic island that harbors both T6SS-1 cluster genes and genes related to STEC pathogenesis. This study reveals a subset of genes present in the Argentine O22:H8 isolates that could confer an advantage over O157:H7 during the colonization process in the bovine intestinal tract.