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<p>A yellow-colored, Gram-stain-negative, non-motile, and rod-shaped bacterium, designated strain H8<sup>T</sup>, was isolated from municipal sludge in Huaibei, China. Strain H8<sup>T</sup> was able to grow at 15–37&#xa0;°C (optimum at 30&#xa0;°C), pH 6.0–8.0 (optimum at pH 7.0), and 0–2.5% (w/v) NaCl concentration (optimum at 0%). This strain was taxonomically characterized by a polyphasic approach. Based on the 16S rRNA gene sequence analysis, strain H8<sup>T</sup> represented to the genus <i>Chitinophaga</i> and shared highest sequence similarities with <i>C. arvensicola</i> DSM 3695<sup>T</sup> (97.5%), <i>C. niastensis</i> JS16-4<sup>T</sup> (97.3%), <i>C. hostae</i> 2R12<sup>T</sup> (97.1%), and <i>C. ginsengisegetis</i> Gsoil 040<sup>T</sup> (96.8%). The 16S rRNA gene similarities with other members of the genus <i>Chitinophaga</i> are less than 96.3%. The only respiratory quinone was a menaquinone with seven isoprene units (MK-7); the major polar lipid was phosphatidylethanolamine; and the predominant fatty acids were iso-C<sub>15:0</sub> and C<sub>16:1</sub> <i>ω</i>5<i>c</i>. The genome size of strain H8<sup>T</sup> was 7.6&#xa0;Mb, with 44.3% G + C content. The DNA–DNA relatedness and the average nucleotide identity values among strain H8<sup>T</sup> and other relatives were all less than 19.7% and 72.6%, respectively, which fall below the threshold value of 70% and 95% for the strain to be considered as novel. The morphological, physiological, chemotaxonomic, and phylogenetic analyses clearly distinguished this strain from its closest phylogenetic neighbors. Thus, strain H8<sup>T</sup> represents a novel species of the genus <i>Chitinophaga</i>, for which the name <i>Chitinophaga defluvii</i> sp. nov. is proposed. The type strain is H8<sup>T</sup> (= CCTCC AB 2023228<sup>T</sup> = KCTC 102174<sup>T</sup>).</p>

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Chitinophaga defluvii sp. nov., a Cyhalofop-Butyl-Degrading Bacterium Isolated from Municipal Sludge

  • 期刊论文

  • 发表日期：

    2025年2月7日

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  • Xinyu Liu,
  • Hailong Liang,
  • Yumeng Zhu,
  • Qian Zhou,
  • Song Shao,
  • Man Song,
  • Huijun Zhang,
  • Xiaomin Wu,
  • Long Zhang

摘要

A yellow-colored, Gram-stain-negative, non-motile, and rod-shaped bacterium, designated strain H8T, was isolated from municipal sludge in Huaibei, China. Strain H8T was able to grow at 15–37 °C (optimum at 30 °C), pH 6.0–8.0 (optimum at pH 7.0), and 0–2.5% (w/v) NaCl concentration (optimum at 0%). This strain was taxonomically characterized by a polyphasic approach. Based on the 16S rRNA gene sequence analysis, strain H8T represented to the genus Chitinophaga and shared highest sequence similarities with C. arvensicola DSM 3695T (97.5%), C. niastensis JS16-4T (97.3%), C. hostae 2R12T (97.1%), and C. ginsengisegetis Gsoil 040T (96.8%). The 16S rRNA gene similarities with other members of the genus Chitinophaga are less than 96.3%. The only respiratory quinone was a menaquinone with seven isoprene units (MK-7); the major polar lipid was phosphatidylethanolamine; and the predominant fatty acids were iso-C15:0 and C16:1 ω5c. The genome size of strain H8T was 7.6 Mb, with 44.3% G + C content. The DNA–DNA relatedness and the average nucleotide identity values among strain H8T and other relatives were all less than 19.7% and 72.6%, respectively, which fall below the threshold value of 70% and 95% for the strain to be considered as novel. The morphological, physiological, chemotaxonomic, and phylogenetic analyses clearly distinguished this strain from its closest phylogenetic neighbors. Thus, strain H8T represents a novel species of the genus Chitinophaga, for which the name Chitinophaga defluvii sp. nov. is proposed. The type strain is H8T (= CCTCC AB 2023228T = KCTC 102174T).