Scholarlink

<p>An aerobic, Gram-stain-positive, motile, coccus-shaped actinomycete, designated strain LSe6-4<sup>T</sup>, was isolated from leaves of sea purslane (<i>Sesuvium portulacastrum</i> L.) in Thailand and subjected to a polyphasic taxonomic studies. Growth of the strain occurred at temperatures between 15 and 38&#xa0;°C, and with NaCl concentrations 0–13%. The 16S rRNA gene sequences analysis indicated that the strain was most closely related to <i>Kineococcus endophyticus</i> KLBMP 1274<sup>T</sup> (99.2%), and <i>Kineococcus mangrovi</i> L2-1-L1<sup>T</sup> (99.0%). The genome of strain LSe6-4<sup>T</sup> was estimated to be 4.22 Mbp in size, with DNA G+C content of 74.57%. The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) relatedness values between strain LSe6-4<sup>T</sup> and the closely related species were less than the threshold values for identifying a novel species. Strain LSe6-4<sup>T</sup> showed morphological and chemotaxonomic characteristics of the genus <i>Kineocoocus</i>. The whole-cell hydrolysates contained <i>meso</i>-diaminopimelic acid as the diagnostic diamino acid, with arabinose, galactose, and ribose as whole-cell sugars. The predominant menaquinone was MK-9(H<sub>2</sub>). The polar lipid profile composed of phosphatidylglycerol, diphosphatidylglycerol, an unidentified phospholipid, three unidentified aminophospholipids, and an unidentified aminolipid. Major cellular fatty acids (&gt; 10% of total fatty acids) were <i>anteiso</i>-C<sub>15:0</sub> and <i>iso</i>-C<sub>14:0</sub>. It is evident from the combination of genotypic and phenotypic characteristics that strain LSe6-4<sup>T</sup> represents a novel species of the genus <i>Kineococcus</i>, for which the name <i>Kineococcus halophytocola</i> sp. nov. is proposed. The type strain is strain LSe6-4<sup>T</sup> (= TBRC 17798<sup>T</sup> = NBRC 116401<sup>T</sup>).</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Kineococcus halophytocola sp. nov., Isolated from Leaves of Halophyte Sesuvium portulacastrum L.

  • 期刊论文

  • 发表日期：

    2025年1月18日

查看全文
  • Jirun Thanompreechachai,
  • Waranya Butdee,
  • Tanatorn Chantavorakit,
  • Chanwit Suriyachadkun,
  • Kannika Duangmal

摘要

An aerobic, Gram-stain-positive, motile, coccus-shaped actinomycete, designated strain LSe6-4T, was isolated from leaves of sea purslane (Sesuvium portulacastrum L.) in Thailand and subjected to a polyphasic taxonomic studies. Growth of the strain occurred at temperatures between 15 and 38 °C, and with NaCl concentrations 0–13%. The 16S rRNA gene sequences analysis indicated that the strain was most closely related to Kineococcus endophyticus KLBMP 1274T (99.2%), and Kineococcus mangrovi L2-1-L1T (99.0%). The genome of strain LSe6-4T was estimated to be 4.22 Mbp in size, with DNA G+C content of 74.57%. The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) relatedness values between strain LSe6-4T and the closely related species were less than the threshold values for identifying a novel species. Strain LSe6-4T showed morphological and chemotaxonomic characteristics of the genus Kineocoocus. The whole-cell hydrolysates contained meso-diaminopimelic acid as the diagnostic diamino acid, with arabinose, galactose, and ribose as whole-cell sugars. The predominant menaquinone was MK-9(H2). The polar lipid profile composed of phosphatidylglycerol, diphosphatidylglycerol, an unidentified phospholipid, three unidentified aminophospholipids, and an unidentified aminolipid. Major cellular fatty acids (> 10% of total fatty acids) were anteiso-C15:0 and iso-C14:0. It is evident from the combination of genotypic and phenotypic characteristics that strain LSe6-4T represents a novel species of the genus Kineococcus, for which the name Kineococcus halophytocola sp. nov. is proposed. The type strain is strain LSe6-4T (= TBRC 17798T = NBRC 116401T).