Background <p>Using stem cells for hair restoration (reversal of hair loss, regeneration of hair follicles) is already a well-established clinical practice.</p> Objective <p>To present regenerative hair transplantation, a pioneering and innovative technique that combines traditional hair transplantation and autologous stem cells to promote hair growth, and improve transplantation results, increasing hair density, hair quality, and graft success rate.</p> Methods <p>Follicular stem cells were obtained in hair transplantation using the regenerative hair transplantation method. After being extracted, the follicles underwent dissection and filtration, and the cell solution obtained was injected into the transplant recipient area, where there was the presence of native follicles.</p> Results <p>Our analysis revealed a high percentage of cells marked with CD44. The combined analysis (cell type and quantity) indicated that most cells had a phenotypic profile characteristic of dermal papilla MSCs<i>.</i></p> Discussion/Conclusion <p>The combination of hair transplantation with the use of autologous mesenchymal cells represents a promising advance in treating baldness. This technique allows the optimization of transplantation results, providing greater hair density. This is because the method combines the clinical treatment of native follicles with autologous cells and the hair transplant. This method is innovative because, to date, there has been no description of this combination in the literature.</p> Level of Evidence IV <p> This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors <a href="http://www.springer.com/00266">www.springer.com/00266</a>.</p>