Scholarlink

<p>Advanced-stage sarcomas pose a major challenge in oncology, as they are often resistant to conventional therapies and associated with poor prognosis. CAR-based cellular immunotherapy is emerging as a very promising therapeutic option; however, clinically relevant animal models are urgently needed to accelerate the clinical development of these approaches. B7-H3 molecule, due to its low expression in normal tissue, high prevalence in multiple human cancers, and association with cancer stemness and aggressiveness, represents one of the most attractive targets for CAR-immunotherapy. In this study, we established a preclinical cellular immunotherapy platform based on canine cytokine-induced killer cells (CIK) redirected by an antihuman B7-H3 CAR against canine sarcoma cells and 3D sarcoma spheroids. B7-H3 was consistently detected across all analyzed canine sarcoma subtypes, including osteosarcoma, soft tissue sarcoma, and hemangiosarcoma, although with variable levels of expression intensity. We successfully generated canine B7-H3-CAR.CIK, achieving a mean CAR expression of 39% ± 2 with an immune phenotype unmodified (NTD) control (CD3 = 92% ± 3, CD8 = 87% ± 7; CD4 = 49% ± 5; CD5 = 77% ± 0.1; NKp46 = 83% ± 5). Canine B7-H3-CAR.CIK efficiently killed canine sarcoma cell lines compared with NTD.CIK, even at low effector/target (E/T) ratios (B7-H3-CAR.CIK: 45% vs 8%; E:T 1:1; 48&#xa0;h; <i>N</i> = 7, <i>n</i> = 8; <i>p</i> &lt; 0.0001), and demonstrated significant cytotoxicity against 3D sarcoma spheroids (58% vs 13%; E:T 2:1; 48&#xa0;h; <i>N</i> = 3, <i>n</i> = 4; <i>p</i> &lt; 0.01). Our findings establish a clinically relevant and translationally valuable platform for evaluating B7-H3-CAR.CIK therapy in dogs with incurable sarcomas, providing a bridge toward the development of novel CAR-based immunotherapies for human incurable sarcomas.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Development and activity of canine B7-H3-CAR.CIK lymphocytes against sarcomas: preclinical evidence and perspectives for human clinical translation

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年9月13日

查看全文
  • Raffaella De Maria,
  • Chiara Donini,
  • Sonia Capellero,
  • Annamaria Massa,
  • Federica Galvagno,
  • Alessia Proment,
  • Letizia Vitali,
  • Elisa Vigna,
  • Elisa Landoni,
  • Gianpietro Dotti,
  • Valeria Leuci,
  • Paolo Accornero,
  • Luiza Cesar Conti,
  • Matteo Olimpo,
  • Enrico Berrino,
  • Giovanni Grignani,
  • Anna Sapino,
  • Dario Sangiolo,
  • Luca Aresu

摘要

Advanced-stage sarcomas pose a major challenge in oncology, as they are often resistant to conventional therapies and associated with poor prognosis. CAR-based cellular immunotherapy is emerging as a very promising therapeutic option; however, clinically relevant animal models are urgently needed to accelerate the clinical development of these approaches. B7-H3 molecule, due to its low expression in normal tissue, high prevalence in multiple human cancers, and association with cancer stemness and aggressiveness, represents one of the most attractive targets for CAR-immunotherapy. In this study, we established a preclinical cellular immunotherapy platform based on canine cytokine-induced killer cells (CIK) redirected by an antihuman B7-H3 CAR against canine sarcoma cells and 3D sarcoma spheroids. B7-H3 was consistently detected across all analyzed canine sarcoma subtypes, including osteosarcoma, soft tissue sarcoma, and hemangiosarcoma, although with variable levels of expression intensity. We successfully generated canine B7-H3-CAR.CIK, achieving a mean CAR expression of 39% ± 2 with an immune phenotype unmodified (NTD) control (CD3 = 92% ± 3, CD8 = 87% ± 7; CD4 = 49% ± 5; CD5 = 77% ± 0.1; NKp46 = 83% ± 5). Canine B7-H3-CAR.CIK efficiently killed canine sarcoma cell lines compared with NTD.CIK, even at low effector/target (E/T) ratios (B7-H3-CAR.CIK: 45% vs 8%; E:T 1:1; 48 h; N = 7, n = 8; p < 0.0001), and demonstrated significant cytotoxicity against 3D sarcoma spheroids (58% vs 13%; E:T 2:1; 48 h; N = 3, n = 4; p < 0.01). Our findings establish a clinically relevant and translationally valuable platform for evaluating B7-H3-CAR.CIK therapy in dogs with incurable sarcomas, providing a bridge toward the development of novel CAR-based immunotherapies for human incurable sarcomas.