Objective <p>To evaluate the possibility of performing renal vessel reconstruction on non-enhanced CT images using deep learning models.</p> Materials and methods <p>177 patients’ CT scans in the non-enhanced phase, arterial phase and venous phase were chosen. These data were randomly divided into the training set (<i>n</i> = 120), validation set (<i>n</i> = 20) and test set (<i>n</i> = 37). In training set and validation set, a radiologist marked out the right renal arteries and veins on non-enhanced CT phase images using contrast phases as references. Trained deep learning models were tested and evaluated on the test set. A radiologist performed renal vessel reconstruction on the test set without the contrast phase reference, and the results were used for comparison. Reconstruction using the arterial phase and venous phase was used as the gold standard.</p> Results <p>Without the contrast phase reference, both radiologist and model could accurately identify artery and vein main trunk. The accuracy was 91.9% vs. 97.3% (model vs. radiologist) in artery and 91.9% vs. 100% in vein, the difference was insignificant. The model had difficulty identify accessory arteries, the accuracy was significantly lower than radiologist (44.4% vs. 77.8%, <i>p</i> = 0.044). The model also had lower accuracy in accessory veins, but the difference was insignificant (64.3% vs. 85.7%, <i>p</i> = 0.094).</p> Conclusion <p>Deep learning models could accurately recognize the right renal artery and vein main trunk, and accuracy was comparable to that of radiologists. Although the current model still had difficulty recognizing small accessory vessels, further training and model optimization would solve these problems.</p>