Scholarlink

Purpose <p>This study evaluated the efficacy of a modified core needle biopsy using a 2&#xa0;cm stroke length to improve tissue yield for molecular analyses in cancer genomics medicine.</p> Methods <p>In vitro biopsy weight evaluations were performed using agarose gels of varying hardness. Ex vivo biopsy weight evaluations were conducted using chicken liver, gizzard, breast, and pork loin tissues. Furthermore, in vivo experiments were performed using murine subcutaneous tumor models. The modified method involved using a coaxial introducer needle to maintain a 1&#xa0;cm notch length while employing a 2&#xa0;cm stroke length to increase tissue yield. Biopsy performance parameters, including sample weight and DNA yield, were compared between the modified and conventional methods. The firing speed of the biopsy needles with 1&#xa0;cm and 2&#xa0;cm stroke&#xa0;lengths was also measured using a high-speed camera.</p> Results <p>The modified technique significantly increased the sample volume across all agarose gel concentrations (9.5–13% increase, P &lt; 0.05). Ex vivo tests revealed significantly higher tissue yields in most samples, except for chicken gizzards. In vivo, the modified method produced significantly larger tissue samples (4.6&#xa0;mg vs. 2.9&#xa0;mg, P &lt; 0.001) and higher DNA yield (8.2&#xa0;ng vs. 6.9&#xa0;ng, P &lt; 0.05). In addition, the biopsy needle’s firing speed was 1.4–1.8 times faster with the 2&#xa0;cm stroke length than 1&#xa0;cm stroke.</p> Conclusion <p>The modified biopsy technique using a 2&#xa0;cm stroke length significantly improved tissue yield, enhancing the quality of biopsy samples for molecular analyses in precision medicine.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Efficacy of modified technique for 1 cm core needle biopsy using a 2 cm cutting length with coaxial introducer needle

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年3月29日

查看全文
  • Hiroki Satomura,
  • Yasushi Kimura,
  • Masahisa Nakamura,
  • Kosuke Tomotake,
  • Koki Yamamoto,
  • Kaishu Tanaka,
  • Yusuke Ono,
  • Hiroki Higashihara,
  • Noriyuki Tomiyama

摘要

Purpose

This study evaluated the efficacy of a modified core needle biopsy using a 2 cm stroke length to improve tissue yield for molecular analyses in cancer genomics medicine.

Methods

In vitro biopsy weight evaluations were performed using agarose gels of varying hardness. Ex vivo biopsy weight evaluations were conducted using chicken liver, gizzard, breast, and pork loin tissues. Furthermore, in vivo experiments were performed using murine subcutaneous tumor models. The modified method involved using a coaxial introducer needle to maintain a 1 cm notch length while employing a 2 cm stroke length to increase tissue yield. Biopsy performance parameters, including sample weight and DNA yield, were compared between the modified and conventional methods. The firing speed of the biopsy needles with 1 cm and 2 cm stroke lengths was also measured using a high-speed camera.

Results

The modified technique significantly increased the sample volume across all agarose gel concentrations (9.5–13% increase, P < 0.05). Ex vivo tests revealed significantly higher tissue yields in most samples, except for chicken gizzards. In vivo, the modified method produced significantly larger tissue samples (4.6 mg vs. 2.9 mg, P < 0.001) and higher DNA yield (8.2 ng vs. 6.9 ng, P < 0.05). In addition, the biopsy needle’s firing speed was 1.4–1.8 times faster with the 2 cm stroke length than 1 cm stroke.

Conclusion

The modified biopsy technique using a 2 cm stroke length significantly improved tissue yield, enhancing the quality of biopsy samples for molecular analyses in precision medicine.