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Purpose <p>Radioligand therapy (RLT) delivers radionuclides, such as Lutetium-177 (<sup>177</sup>Lu), to selectively target cancer cells. Despite the favourable clinical outcomes of <sup>177</sup>Lu-based RLT, complete cure remains infrequent. A comprehensive understanding of its mode of action (MoA) is essential for strategic design of novel combination approaches that could improve therapeutic responses.</p> Methods <p>DNA damage response (DDR) markers were profiled by immunofluorescence in cancer cell lines treated with DOTA-chelated <sup>177</sup>Lu ([<sup>177</sup>Lu]Lu-DOTA). Multiple DDR-deficient isogenic cell lines and DDR inhibitors were screened in viability assays with [<sup>177</sup>Lu]Lu-DOTA to identify sensitizers. <sup>177</sup>Lu-RLT combination with an inhibitor of the non-homologous end joining (NHEJ) core factor DNA-PK, and its effect on viability and cell death, were further characterised in vitro and in vivo.</p> Results <p>[<sup>177</sup>Lu]Lu-DOTA treatment induced multiple DDR biomarkers indicative of DNA double-strand break (DSB) repair and genomic instability. Viability assays performed with DDR-deficient isogenic models and DDR inhibitors demonstrated that loss of DSB repair through NHEJ elicits the strongest sensitisation to [<sup>177</sup>Lu]Lu-DOTA. Consistently, DNA-PK inhibition strongly sensitised PSMA-positive prostate cancer cells to [<sup>177</sup>Lu]Lu-PSMA-617, exacerbated apoptosis and cell cycle arrest and improved efficacy in vivo, demonstrating good tolerability.</p> Conclusion <p>These results provide novel mechanistic insights into <sup>177</sup>Lu-based RLT and pinpoint NHEJ as a key pathway for the repair of <sup>177</sup>Lu-induced DNA damage. DNA-PK inhibition strongly sensitises cancer cells to <sup>177</sup>Lu-based RLT and results in stronger potentiation compared with other tested DDR inhibitor combinations, warranting the clinical exploration of <sup>177</sup>Lu-based RLT and DNA-PK inhibitor (DNA-PKi) combination.</p>

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Deciphering the phenotypic responses to 177Lu-based radioligand therapy to identify efficacious combinations

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年7月23日

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  • Marco Ranzani,
  • Alessandro Cicconi,
  • Guillermo Sastre-Moreno,
  • Asmita Thapa,
  • Elise Simon,
  • Josefine Reber,
  • Joseph Barlow,
  • Marie Laure Boursier,
  • Aurora Cerutti,
  • Luisa Deberle,
  • Luigi Del Pozzo,
  • Elias Elinati,
  • Lerin Geo,
  • Vera Grinkevich,
  • Charlene Hartnagel,
  • Sravanth K. Hindupur,
  • Katjusa Koler,
  • Suraj Menon,
  • Joana Neves,
  • Barbara Schacher,
  • Nan Shao,
  • Eeson Rajendra,
  • Markus Reschke,
  • Tobias Schmelzle,
  • Graeme C. M. Smith,
  • Helen M. R. Robinson,
  • Marta Cortes-Cros

摘要

Purpose

Radioligand therapy (RLT) delivers radionuclides, such as Lutetium-177 (177Lu), to selectively target cancer cells. Despite the favourable clinical outcomes of 177Lu-based RLT, complete cure remains infrequent. A comprehensive understanding of its mode of action (MoA) is essential for strategic design of novel combination approaches that could improve therapeutic responses.

Methods

DNA damage response (DDR) markers were profiled by immunofluorescence in cancer cell lines treated with DOTA-chelated 177Lu ([177Lu]Lu-DOTA). Multiple DDR-deficient isogenic cell lines and DDR inhibitors were screened in viability assays with [177Lu]Lu-DOTA to identify sensitizers. 177Lu-RLT combination with an inhibitor of the non-homologous end joining (NHEJ) core factor DNA-PK, and its effect on viability and cell death, were further characterised in vitro and in vivo.

Results

[177Lu]Lu-DOTA treatment induced multiple DDR biomarkers indicative of DNA double-strand break (DSB) repair and genomic instability. Viability assays performed with DDR-deficient isogenic models and DDR inhibitors demonstrated that loss of DSB repair through NHEJ elicits the strongest sensitisation to [177Lu]Lu-DOTA. Consistently, DNA-PK inhibition strongly sensitised PSMA-positive prostate cancer cells to [177Lu]Lu-PSMA-617, exacerbated apoptosis and cell cycle arrest and improved efficacy in vivo, demonstrating good tolerability.

Conclusion

These results provide novel mechanistic insights into 177Lu-based RLT and pinpoint NHEJ as a key pathway for the repair of 177Lu-induced DNA damage. DNA-PK inhibition strongly sensitises cancer cells to 177Lu-based RLT and results in stronger potentiation compared with other tested DDR inhibitor combinations, warranting the clinical exploration of 177Lu-based RLT and DNA-PK inhibitor (DNA-PKi) combination.