Purpose <p>In this trial we explore potential indications of an intra-operative mobile PET/CT camera. The tested device is designed to acquire high quality images of resected tissue specimens from patients who were administered a PET-tracer, shortly before resection. Besides clinical experiences, we will also comment on the practical aspects of the implementation of a safe workflow for intra-operative PET/CT-imaging.</p> Methods <p>This investigator driven study involved a 12-month evaluation of the AURA 10 PET/CT camera (XEOS Medical, Belgium). Depending on the tumour type, [¹⁸F]FDG, [18F]JK-PSMA-7, [^18F]PSMA-1007 or [¹⁸F]Choline was injected intravenously 60–90&#xa0;min prior to the removal of the tumour. The tissue was scanned in the mobile PET/CT-device and 12&#xa0;min later the surgeon could review the images. Specimen PET/CT-images were confronted with pathology findings. Dose rates were monitored around the patient throughout the procedure.</p> Results <p>The technique was tested in 32 surgeries for thyroid carcinomas (<i>n</i> = 5), transitional cell carcinomas (<i>n</i> = 2,) renal cell carcinoma (<i>n</i> = 1), prostate cancer (<i>n</i> = 5), breast carcinoma (<i>n</i> = 7), skin cancer (<i>n</i> = 3), nodal or bone biopsy for oncology work up (<i>n</i> = 6) and parathyroid adenoma (<i>n</i> = 3). Normalized to an injected activity of 1 MBq/kg the estimated median absorbed doses per procedure were 15.6 µSv (range 0,7-140,8) and 14,1 µSv (0,5–46,2) for respectively the surgeons and instrumenting nurses.</p> Conclusion <p>The overall experience of intra-operative PET/CT-imaging of surgical specimens was promising in our hospital, with particular added value in case of (para)thyroid, urological surgeries, and oncological work-ups. High quality images were obtained with low activity of tracers, enabling a safe implementation.</p> Trial registration number (Belgium) <p>BUN: B0172022000009 (NCT retrospectively submitted).</p>