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Abstract <p>Since plastics pose the greatest threat to humanity, it is essential to find an economic and sustainable solution to combat environmental pollution. In this study, the ability of polyhydroxyalkanoates (PHA) production by the halophilic bacterium <i>Halovirbrio</i> sp. HP20-59 in the presence of different carbon sources was examined. The strain showed a selective substrate preference, with the highest PHA production (reaching up to 73% of cell dry weight) in the presence of galactose, while fructose, arabinose, glycerol and xylose resulted in lower accumulation. Phylogenetic analysis based on the&#xa0;16S rRNA gene sequence and whole-genome sequencing confirmed the HP20-59 strain as a novel species within the <i>Oceanospirillales</i> order. Draft genome showed a size of 4,165,370&#xa0;bp with a GC content of 55.1% and a complete set of <i>pha</i> genes. The comparative analysis of the <i>phaC</i> gene identified a 638 amino acid-long class I poly(R)-hydroxyalkanoic acid synthase, showing 91% similarity to <i>Halovibrio variabilis</i> and 89% similarity to species within the <i>Vreelandella</i> genus, suggesting a possible horizontal gene transfer of the <i>pha</i> gene cluster. These findings highlight the unique genetic and metabolic characteristics of <i>Halovibrio</i> sp. HP20-59, making it a promising candidate for industrial PHA production and a valuable resource for research on sustainable biopolymers.</p> Key points <p><UnorderedList Mark="Bullet"> <ItemContent> <p><i>The first study of PHB production by the halophilic Halovibrio spp.</i></p> </ItemContent> <ItemContent> <p><i>The highest level of PHB production observed using glucose, galactose, and sucrose.</i></p> </ItemContent> <ItemContent> <p><i>phaCAB operon possibly acquired by horizontal gene transfer from Vreelandella sp.</i></p> </ItemContent> </UnorderedList></p>

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Genome sequence of Halovibrio sp. HP20-59 as a promising polyhydroxybutyrate producer

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年1月10日

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  • Shivani Adhvaryu,
  • Jana Kiskova,
  • Maria Piknova,
  • Veronika Farkasova,
  • Iva Buchtikova,
  • Xenie Kourilova,
  • Martin Kizovsky,
  • Marketa Benesova,
  • Ota Samek,
  • Stanislav Obruca,
  • Peter Pristas

摘要

Abstract

Since plastics pose the greatest threat to humanity, it is essential to find an economic and sustainable solution to combat environmental pollution. In this study, the ability of polyhydroxyalkanoates (PHA) production by the halophilic bacterium Halovirbrio sp. HP20-59 in the presence of different carbon sources was examined. The strain showed a selective substrate preference, with the highest PHA production (reaching up to 73% of cell dry weight) in the presence of galactose, while fructose, arabinose, glycerol and xylose resulted in lower accumulation. Phylogenetic analysis based on the 16S rRNA gene sequence and whole-genome sequencing confirmed the HP20-59 strain as a novel species within the Oceanospirillales order. Draft genome showed a size of 4,165,370 bp with a GC content of 55.1% and a complete set of pha genes. The comparative analysis of the phaC gene identified a 638 amino acid-long class I poly(R)-hydroxyalkanoic acid synthase, showing 91% similarity to Halovibrio variabilis and 89% similarity to species within the Vreelandella genus, suggesting a possible horizontal gene transfer of the pha gene cluster. These findings highlight the unique genetic and metabolic characteristics of Halovibrio sp. HP20-59, making it a promising candidate for industrial PHA production and a valuable resource for research on sustainable biopolymers.

Key points

The first study of PHB production by the halophilic Halovibrio spp.

The highest level of PHB production observed using glucose, galactose, and sucrose.

phaCAB operon possibly acquired by horizontal gene transfer from Vreelandella sp.