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Abstract <p>Administering beneficial bacteria as probiotics to restore the intestinal microbiota and its metabolic functions, such as butyrogenesis, is a promising treatment strategy in ulcerative colitis (UC). This study aimed to investigate the effect of a combination of probiotics, consisting of the lactic acid bacterium <i>Weizmannia coagulans</i> SANK70258 and the lactate-utilizing butyrate-producing bacteria <i>Anaerostipes caccae</i> or <i>Clostridium butyricum</i>, on the colonic environment using an in vitro colonic microbiota culture model with fecal inoculums from seven patients with UC. Co-inoculated <i>W. coagulans</i> and <i>A. caccae</i> neither inhibited each other’s growth nor significantly affected the relative abundance of other bacterial species; however, the growth of <i>W. coagulans</i> was significantly inhibited when co-inoculated with <i>C. butyricum</i>. The relative abundance of pro-inflammatory bacteria (<i>Escherichia</i> sp. and unclassified <i>Enterobacteriaceae</i>) and <i>Bifidobacterium</i> spp. significantly decreased in <i>W. coagulans</i>-<i>C. butyricum</i> co-inoculated cultures. Inoculation with any of the probiotics alone did not increase butyrate production, whereas co-inoculation of <i>W. coagulans</i> with <i>A. caccae</i> or <i>C. butyricum</i> significantly increased the butyrate levels. Overall, the results suggested that <i>W. coagulans</i> and lactate-utilizing butyrate-producing bacteria in combination have synergistic effects through cross-feeding and can effectively restore butyrogenesis in the colonic environment of patients with UC.</p> Key points <p><i>• Effects of probiotics were evaluated using in vitro microbiota model of UC colon.</i></p> <p><i>• W. coagulans and lactate-utilizing butyrate producers have synergistic effects.</i></p> <p><i>• Co-inoculation of W. coagulans with A. caccae or C. butyricum enhanced butyrogenesis.</i></p> Graphical Abstract <p></p>

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Combination of probiotics enhancing butyrogenesis in colonic microbiota model of patients with ulcerative colitis

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年5月10日

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  • Kentaro Inokuma,
  • Daisuke Sasaki,
  • Tomoya Shintani,
  • Jun Inoue,
  • Katsuaki Oyama,
  • Yuta Noda,
  • Takayuki Maeda,
  • Ryouichi Yamada,
  • Yasushi Matsuki,
  • Yuzo Kodama,
  • Akihiko Kondo

摘要

Abstract

Administering beneficial bacteria as probiotics to restore the intestinal microbiota and its metabolic functions, such as butyrogenesis, is a promising treatment strategy in ulcerative colitis (UC). This study aimed to investigate the effect of a combination of probiotics, consisting of the lactic acid bacterium Weizmannia coagulans SANK70258 and the lactate-utilizing butyrate-producing bacteria Anaerostipes caccae or Clostridium butyricum, on the colonic environment using an in vitro colonic microbiota culture model with fecal inoculums from seven patients with UC. Co-inoculated W. coagulans and A. caccae neither inhibited each other’s growth nor significantly affected the relative abundance of other bacterial species; however, the growth of W. coagulans was significantly inhibited when co-inoculated with C. butyricum. The relative abundance of pro-inflammatory bacteria (Escherichia sp. and unclassified Enterobacteriaceae) and Bifidobacterium spp. significantly decreased in W. coagulans-C. butyricum co-inoculated cultures. Inoculation with any of the probiotics alone did not increase butyrate production, whereas co-inoculation of W. coagulans with A. caccae or C. butyricum significantly increased the butyrate levels. Overall, the results suggested that W. coagulans and lactate-utilizing butyrate-producing bacteria in combination have synergistic effects through cross-feeding and can effectively restore butyrogenesis in the colonic environment of patients with UC.

Key points

• Effects of probiotics were evaluated using in vitro microbiota model of UC colon.

• W. coagulans and lactate-utilizing butyrate producers have synergistic effects.

• Co-inoculation of W. coagulans with A. caccae or C. butyricum enhanced butyrogenesis.

Graphical Abstract