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<p><i>Acantholyda posticalis</i> (Matsumura) is a globally significant forest pest that inflicts substantial economic losses through its feeding activity on <i>Pinus</i> species. As an oligophagous insect, <i>A. posticalis</i> relies critically on its gut microbiota to overcome the defensive secondary metabolites of pine needles, particularly α- and β-pinene terpenoids. This study investigated the dynamic compositional changes of gut bacterial communities across different developmental stages of <i>A. posticalis</i> and characterized their functional roles in host adaptation. Through traditional culturing methods, two pinene-degrading bacterial strains—<i>Klebsiella variicola</i> and <i>Enterobacter hormaechei</i>—were isolated from the larval gut. In vitro assays demonstrated their significant capacity to degrade the two pinenes. High-throughput 16S rRNA sequencing revealed stage-specific bacterial enrichment patterns. Functional prediction suggested these microbial communities participate in critical metabolic processes, including phosphotransferase systems, GST activity, and detoxification pathways. This work advances understanding of insect-microbe symbiosis in oligophagous systems and proposes novel strategies for ecologically sustainable <i>A. posticalis</i> control through manipulation of its gut microbiota.</p>

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Microbial Mediators of Pine Defense Resistance: Stage-Specific Gut Symbionts Enable Acantholyda posticalis to Overcome Terpenoid Barriers

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年1月31日

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  • Yao Wang,
  • Shipeng Han,
  • Wenlong Zhang,
  • Weixing Shen,
  • Bin Dong,
  • Ningxin Wang

摘要

Acantholyda posticalis (Matsumura) is a globally significant forest pest that inflicts substantial economic losses through its feeding activity on Pinus species. As an oligophagous insect, A. posticalis relies critically on its gut microbiota to overcome the defensive secondary metabolites of pine needles, particularly α- and β-pinene terpenoids. This study investigated the dynamic compositional changes of gut bacterial communities across different developmental stages of A. posticalis and characterized their functional roles in host adaptation. Through traditional culturing methods, two pinene-degrading bacterial strains—Klebsiella variicola and Enterobacter hormaechei—were isolated from the larval gut. In vitro assays demonstrated their significant capacity to degrade the two pinenes. High-throughput 16S rRNA sequencing revealed stage-specific bacterial enrichment patterns. Functional prediction suggested these microbial communities participate in critical metabolic processes, including phosphotransferase systems, GST activity, and detoxification pathways. This work advances understanding of insect-microbe symbiosis in oligophagous systems and proposes novel strategies for ecologically sustainable A. posticalis control through manipulation of its gut microbiota.