Scholarlink

<p>Biofilm formation is a survival strategy for bacteria, contributing to their persistence in natural and industrial environments. In this study, we investigated the ability of extracellular products (ECPs) produced by the probiotic strain <i>Shewanella</i> sp<i>.</i> Pdp11 under different culture conditions to inhibit biofilm formation in pathogenic and environmental <i>Shewanella</i> strains. ECPs from specific culture conditions altered biofilm formation in several <i>Shewanella</i> strains, with <i>Shewanella hafniensis</i> P14 displaying the highest sensitivity. Metabolomic analysis of the ECPs identified glycogen as a key metabolite associated with biofilm inhibition. Further genomic analysis of <i>S</i>. <i>hafniensis</i> P14 revealed an interruption in its glycogen synthesis pathway, suggesting a dependency on external glycogen-related metabolites for biofilm development. These findings demonstrate that <i>Shewanella</i> sp. Pdp11 ECPs can modify biofilm formation across multiple <i>Shewanella</i> strains, particularly in <i>S</i>. <i>hafniensis</i> P14 through glycogen-associated mechanisms.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Metabolite-Driven Modulation of Biofilm Formation in Shewanella: Insights from Shewanella sp. Pdp11 Extracellular Products

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年5月27日

查看全文
  • Olivia Pérez-Gómez,
  • Marta Domínguez-Maqueda,
  • Jorge García-Márquez,
  • Miguel Ángel Moriñigo,
  • Silvana T. Tapia-Paniagua

摘要

Biofilm formation is a survival strategy for bacteria, contributing to their persistence in natural and industrial environments. In this study, we investigated the ability of extracellular products (ECPs) produced by the probiotic strain Shewanella sp. Pdp11 under different culture conditions to inhibit biofilm formation in pathogenic and environmental Shewanella strains. ECPs from specific culture conditions altered biofilm formation in several Shewanella strains, with Shewanella hafniensis P14 displaying the highest sensitivity. Metabolomic analysis of the ECPs identified glycogen as a key metabolite associated with biofilm inhibition. Further genomic analysis of S. hafniensis P14 revealed an interruption in its glycogen synthesis pathway, suggesting a dependency on external glycogen-related metabolites for biofilm development. These findings demonstrate that Shewanella sp. Pdp11 ECPs can modify biofilm formation across multiple Shewanella strains, particularly in S. hafniensis P14 through glycogen-associated mechanisms.