Scholarlink

Background <p>Human lipoaspirate (HL), long regarded as surgical waste, is a rich reservoir of regenerative elements including stromal vascular fraction (SVF), adipose-derived stem cells (ADSCs), extracellular matrix (ECM) fragments, and soluble paracrine mediators. The liposuction process itself represents the first mechanical dissociation of adipose tissue, generating an infranatant fraction that contains both cellular and acellular bioactive components. Conventional fat-processing methods—focused primarily on aesthetic graft purification—typically discard this liquid phase, resulting in substantial loss of regenerative potential.</p> Methods <p>The Waste-free Intelligent Stromal Vascular Fraction Extraction (WISE) protocol introduces a systematic, fully mechanical workflow designed to maximize recovery from HL within intraoperative conditions. It combines gravitational separation, shear-based dissociation, and sequential centrifugation to isolate five reproducible fractions: oil, fat, liquid, water, and pellet phases. Each phase contains distinct biological components that are characterized macroscopically and microscopically from over 20 intraoperative samples, with biochemical profiles correlated to the literature.</p> Results <p>WISE consistently produced highly reproducible fractions containing viable stromal cells, red blood cells, cytokines, extracellular vesicles, and ECM fragments—without the need for enzymatic digestion. The protocol’s dual-pathway design accommodates both large- and small-volume procedures. Comparative studies show that optimized mechanical parameters—such as 20 intersyringe passages, vibration-based dissociation at 30 Hz for 15 min, and three-step mechanical digestion—achieve nucleated cell yields and viability rates (&gt;85%) comparable to enzymatic methods while maintaining regulatory compliance with FDA and intraoperative feasibility.</p> Conclusions <p>By reframing liposuction as the initial mechanical dissociation event and systematically recovering all derivative phases, the WISE protocol transforms HL into a complete regenerative bioresource. This no-waste, standardized, and enzyme-free strategy enhances therapeutic yield while ensuring clinical safety, reproducibility, and alignment with minimal manipulation standards.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

WISE: A systematic mechanical protocol for maximizing therapeutic product yield from human lipoaspirate

  • 期刊论文

  • 发表日期：

    2026年1月15日

查看全文
  • Hebert Lamblet

摘要

Background

Human lipoaspirate (HL), long regarded as surgical waste, is a rich reservoir of regenerative elements including stromal vascular fraction (SVF), adipose-derived stem cells (ADSCs), extracellular matrix (ECM) fragments, and soluble paracrine mediators. The liposuction process itself represents the first mechanical dissociation of adipose tissue, generating an infranatant fraction that contains both cellular and acellular bioactive components. Conventional fat-processing methods—focused primarily on aesthetic graft purification—typically discard this liquid phase, resulting in substantial loss of regenerative potential.

Methods

The Waste-free Intelligent Stromal Vascular Fraction Extraction (WISE) protocol introduces a systematic, fully mechanical workflow designed to maximize recovery from HL within intraoperative conditions. It combines gravitational separation, shear-based dissociation, and sequential centrifugation to isolate five reproducible fractions: oil, fat, liquid, water, and pellet phases. Each phase contains distinct biological components that are characterized macroscopically and microscopically from over 20 intraoperative samples, with biochemical profiles correlated to the literature.

Results

WISE consistently produced highly reproducible fractions containing viable stromal cells, red blood cells, cytokines, extracellular vesicles, and ECM fragments—without the need for enzymatic digestion. The protocol’s dual-pathway design accommodates both large- and small-volume procedures. Comparative studies show that optimized mechanical parameters—such as 20 intersyringe passages, vibration-based dissociation at 30 Hz for 15 min, and three-step mechanical digestion—achieve nucleated cell yields and viability rates (>85%) comparable to enzymatic methods while maintaining regulatory compliance with FDA and intraoperative feasibility.

Conclusions

By reframing liposuction as the initial mechanical dissociation event and systematically recovering all derivative phases, the WISE protocol transforms HL into a complete regenerative bioresource. This no-waste, standardized, and enzyme-free strategy enhances therapeutic yield while ensuring clinical safety, reproducibility, and alignment with minimal manipulation standards.