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Background <p>Estrogen deficiency, a key factor in osteoporosis, may influence peri-implant healing, which requires cautious and individualized planning for dental implant placement. Functionalizing biomaterials with bioactive molecules, such as soy-derived isoflavone, could enhance their osteoconductive potential.</p> Objective <p>Investigate the performance of genistein in the functionalization of bioactive glass and deproteinized bovine bone by sonochemistry on peri-implant bone repair in ovariectomized rats.</p> Methods <p>In total, 50 female rats were divided into five groups (<i>n</i> = 10): blood clot (CLOT); bioactive glass (BG); bioactive glass functionalized with genistein (BG + GEN); deproteinized bovine bone (BO); and deproteinized bovine bone functionalized with genistein (BO + GEN). Thirty days after ovariectomy, implants were placed in the tibia of the rats. Calcein and alizarin were administered at 14 and 24 days post-surgery, respectively. The rats were euthanized 28 days after implant placement for analysis.</p> Results <p>BO + GEN showed the highest removal torque (5.4 Ncm), significantly higher than BO (2.3 Ncm; <i>p</i> &lt; 0.05), followed by BG + GEN (4.2 Ncm), BG (3.3 Ncm), and CLOT (2.1 Ncm). BG + GEN and BO + GEN upregulated the bone markers (RANKL, OPG, OCN, IBSP) (<i>p</i> &lt; 0.05). Micro-CT revealed higher bone volume percentage (BV/TV) in BO + GEN (11.67%) and BG + GEN (10.51%) versus their controls (<i>p</i> &lt; 0.05). Trabecular separation (Tb.Sp) was significantly reduced in BO + GEN compared to BG (<i>p</i> &lt; 0.05).</p> Conclusion <p>These findings suggest that genistein can potentiate the osteoconductive properties of clinically used biomaterials, enhancing peri-implant bone repair in ovariectomized rats.</p>

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Local Delivery of Genistein in Peri-Implant Defects Enhances Osseointegration in Ovariectomized Rats

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年2月17日

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  • Nathália Dantas Duarte,
  • Fábio Roberto de Souza Batista,
  • Marcelly Braga Gomes,
  • Laura Gabriela Macedo,
  • Naara Gabriela Monteiro,
  • Gabriel Mulinari-Santos,
  • Pedro Henrique Silva Gomes-Ferreira,
  • Paulo Noronha Lisboa-Filho,
  • Luiz Meirelles,
  • Roberta Okamoto

摘要

Background

Estrogen deficiency, a key factor in osteoporosis, may influence peri-implant healing, which requires cautious and individualized planning for dental implant placement. Functionalizing biomaterials with bioactive molecules, such as soy-derived isoflavone, could enhance their osteoconductive potential.

Objective

Investigate the performance of genistein in the functionalization of bioactive glass and deproteinized bovine bone by sonochemistry on peri-implant bone repair in ovariectomized rats.

Methods

In total, 50 female rats were divided into five groups (n = 10): blood clot (CLOT); bioactive glass (BG); bioactive glass functionalized with genistein (BG + GEN); deproteinized bovine bone (BO); and deproteinized bovine bone functionalized with genistein (BO + GEN). Thirty days after ovariectomy, implants were placed in the tibia of the rats. Calcein and alizarin were administered at 14 and 24 days post-surgery, respectively. The rats were euthanized 28 days after implant placement for analysis.

Results

BO + GEN showed the highest removal torque (5.4 Ncm), significantly higher than BO (2.3 Ncm; p < 0.05), followed by BG + GEN (4.2 Ncm), BG (3.3 Ncm), and CLOT (2.1 Ncm). BG + GEN and BO + GEN upregulated the bone markers (RANKL, OPG, OCN, IBSP) (p < 0.05). Micro-CT revealed higher bone volume percentage (BV/TV) in BO + GEN (11.67%) and BG + GEN (10.51%) versus their controls (p < 0.05). Trabecular separation (Tb.Sp) was significantly reduced in BO + GEN compared to BG (p < 0.05).

Conclusion

These findings suggest that genistein can potentiate the osteoconductive properties of clinically used biomaterials, enhancing peri-implant bone repair in ovariectomized rats.