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<p>The sandwich amperometric immunosensor based on pequi- derived carbon dots (CDs) for labeling second antibody represents a promising approach for a rapid and quantitative detection of <i>Salmonella</i> sp. in liquid (water used for washing chicken- CWW) and solid (cheese- CC1) samples. The original microbiota of each sample was preserved, and each one was intentionally contaminated with <i>Salmonella enterica</i> serovar Typhimurium. The immunosensor was tested in the presence of <i>Escherichia coli</i> and <i>Staphylococcus aureus</i>, it exhibited specificity and effectively detected <i>Salmonella</i> with a low limit of detection (LOD) which was 0.8 CFU/mL and 0.1 CFU/mL for CWW and CC1, respectively. The electrochemical measurement time was two minutes without pre-enrichment step of the pathogen and the total assay time was about two hours. This study demonstrates the potential of this immunosensor for a rapid and sensitive <i>Salmonella</i> detection in real samples.</p>

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Detection of Salmonella typhimurium in food matrices by an amperometric immunosensor with Pequi carbon dot- labeled antibody

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年3月3日

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  • Rachel Menezes Castelo,
  • Marília de Albuquerque Oliveira,
  • Denilson Oliveira Menezes,
  • Ruan Sousa de Santana,
  • Airis Maria Araújo Melo,
  • Roselayne Ferro Furtado,
  • Terezinha Feitosa Machado,
  • Carlucio Roberto Alves

摘要

The sandwich amperometric immunosensor based on pequi- derived carbon dots (CDs) for labeling second antibody represents a promising approach for a rapid and quantitative detection of Salmonella sp. in liquid (water used for washing chicken- CWW) and solid (cheese- CC1) samples. The original microbiota of each sample was preserved, and each one was intentionally contaminated with Salmonella enterica serovar Typhimurium. The immunosensor was tested in the presence of Escherichia coli and Staphylococcus aureus, it exhibited specificity and effectively detected Salmonella with a low limit of detection (LOD) which was 0.8 CFU/mL and 0.1 CFU/mL for CWW and CC1, respectively. The electrochemical measurement time was two minutes without pre-enrichment step of the pathogen and the total assay time was about two hours. This study demonstrates the potential of this immunosensor for a rapid and sensitive Salmonella detection in real samples.