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<p>The aim of this study was to isolate and characterize lactic acid bacteria (LAB) producing exopolysaccharides (EPS) from kefir and to optimize the cultivation conditions using a Rotatable Central Composite Design (RCCD) to increase biopolymer production. From kefir grains (five samples) and the fermented milk obtained, 44 LAB isolates were selected, of which eight were considered microbiologically safe and exhibited desirable technological characteristics. Isolate KLM6, molecularly identified as <i>Leuconostoc mesenteroides</i>, was selected for cultivation condition optimization, resulting in a 105.1% increase in EPS yield compared to previous results, using standard fermentation parameters (pH 6.2 and incubation at 37&#xa0;°C for 24&#xa0;h). This increase was due to the interplay of pH and temperature variables (pH 5.0 and incubation at 50.5&#xa0;°C for 24&#xa0;h), achieving a maximum EPS concentration of 0.841&#xa0;g/L. It was concluded that alterations in cultivation conditions were determinative for the synthesis of this biopolymer, enabling the efficient future application of <i>L. mesenteroides</i> KLM6 in the food industry, since EPS-producing LAB are valued for improving the texture and viscosity of fermented products.</p>

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Exopolysaccharides produced by lactic acid bacteria from kefir: production and optimization of cultivation conditions

  • 期刊论文

  • 发表日期：

    2025年10月6日

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  • Caroline Krause Bierhals,
  • Maria Fernanda Fernandes Siqueira,
  • Silvana de Souza Sigali,
  • Isabela Schneid Kroning,
  • Igor Henrique de Lima Costa,
  • Khadija Bezerra Massaut,
  • Graciela Volz Lopes,
  • Wladimir Padilha da Silva,
  • Ângela Maria Fiorentini

摘要

The aim of this study was to isolate and characterize lactic acid bacteria (LAB) producing exopolysaccharides (EPS) from kefir and to optimize the cultivation conditions using a Rotatable Central Composite Design (RCCD) to increase biopolymer production. From kefir grains (five samples) and the fermented milk obtained, 44 LAB isolates were selected, of which eight were considered microbiologically safe and exhibited desirable technological characteristics. Isolate KLM6, molecularly identified as Leuconostoc mesenteroides, was selected for cultivation condition optimization, resulting in a 105.1% increase in EPS yield compared to previous results, using standard fermentation parameters (pH 6.2 and incubation at 37 °C for 24 h). This increase was due to the interplay of pH and temperature variables (pH 5.0 and incubation at 50.5 °C for 24 h), achieving a maximum EPS concentration of 0.841 g/L. It was concluded that alterations in cultivation conditions were determinative for the synthesis of this biopolymer, enabling the efficient future application of L. mesenteroides KLM6 in the food industry, since EPS-producing LAB are valued for improving the texture and viscosity of fermented products.