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<p>In order to effectively extract polyphenols from <i>camellia</i> seed meal in a green and efficient manner, an environmentally friendly and efficient method based on ultrasound-assisted cold isostatic pressure extraction was established. The optimum extraction conditions were determined by one-way and Box-Behnken Design experiments: pressure of 423.428&#xa0;MPa, pressure maintained for 15.536&#xa0;min, <i>camellia</i> seed meal addition of 10.578%, and ethanol concentration of 78.218%. Qualitative and quantitative analyses of the extracted polyphenols were carried out using LC-MS/MS, and the results showed that higher extraction rates could be obtained using CIP and ultrasonic co-processing. Based on the analysis of the results of FTIR, XRD and SEM spectra, the synergistic pretreatment with cold isostatic pressure and ultrasound destroyed the microstructure of the plant, and the active substance flows out more easily. Finally, in vitro antioxidant assay, anti-inflammatory assay and bacteriostatic assay were used to test the activity of the extracts. The results showed that the combined treatment of cold isostatic pressure and ultrasound did not destroy the activity of the extracts. Compared with the blank treatment group, the ultrasound assisted cold isostatic pressure treatment group showed a 27% increase in ABTS free radical scavenging ability and an approximately 20% increase in the ability to inhibit TNF - α and IL-6. This study established a green extraction method without chemical additives and provided a green method for the utilization of <i>camellia</i> seed meal.</p>

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Study on green extraction process of polyphenols from camellia seed meal and determination of extract bioactivity

  • 期刊论文

  • 发表日期：

    2025年9月26日

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  • Jing Wang,
  • Ming Lang,
  • Xueting Sang,
  • Tiantian Zhou,
  • Zhiyun Zhang,
  • Bin Qu,
  • Yuxin Wang,
  • Feng Zhen

摘要

In order to effectively extract polyphenols from camellia seed meal in a green and efficient manner, an environmentally friendly and efficient method based on ultrasound-assisted cold isostatic pressure extraction was established. The optimum extraction conditions were determined by one-way and Box-Behnken Design experiments: pressure of 423.428 MPa, pressure maintained for 15.536 min, camellia seed meal addition of 10.578%, and ethanol concentration of 78.218%. Qualitative and quantitative analyses of the extracted polyphenols were carried out using LC-MS/MS, and the results showed that higher extraction rates could be obtained using CIP and ultrasonic co-processing. Based on the analysis of the results of FTIR, XRD and SEM spectra, the synergistic pretreatment with cold isostatic pressure and ultrasound destroyed the microstructure of the plant, and the active substance flows out more easily. Finally, in vitro antioxidant assay, anti-inflammatory assay and bacteriostatic assay were used to test the activity of the extracts. The results showed that the combined treatment of cold isostatic pressure and ultrasound did not destroy the activity of the extracts. Compared with the blank treatment group, the ultrasound assisted cold isostatic pressure treatment group showed a 27% increase in ABTS free radical scavenging ability and an approximately 20% increase in the ability to inhibit TNF - α and IL-6. This study established a green extraction method without chemical additives and provided a green method for the utilization of camellia seed meal.