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<p>This study aimed to investigate the effects of Atmospheric cold plasma (ACP) treatment on microbial communities and ACP-induced oxidation on proteins in crab paste during 6-day storage at 4&#xa0;°C. 16&#xa0;S ribosomal RNA (16&#xa0;S rRNA) gene amplicon sequencing was used to analyze changes in the bacterial community of the crab paste through indices including bacterial composition, alpha diversity, beta diversity, and Linear discriminant analysis Effect Size (LEfSe) analysis, while protein changes were assessed by examining the secondary structure and allergenic protein content. The results demonstrated that ACP-treated samples exhibited significantly reduced microbial indices compared to the control group. <i>Vibrio</i> emerged as the predominant species in both groups during the storage. Compared with the control group, ACP treatment eliminated most bacterial genera, including <i>Variovorax</i>, <i>Vibrionimonas</i>, <i>Bradyrhizobium</i>, <i>Burkholderia-Caballeronia-Paraburkholderia</i>, <i>Hydrogenophaga</i>, and <i>Pseudomonas</i>. Functional prediction analysis indicated that ACP treatment and storage conditions substantially affected metabolic pathways, especially those associated with carbohydrate, amino acid, and energy metabolism. Besides, oxidation induced by ACP treatment- led to substantial alterations in the secondary structure of myofibrillar protein. ACP treatment increased random coil content by 83.33%, while reducing α-helix, β-turn, and β-sheet by 4.88%, 14.29%, and 21.05%, respectively. The content of allergenic proteins, like tropomyosin (TM) and arginine kinase (AK), decreased by 24.31% (from 38.54 to 29.17 ng/g) and 35.35% (from 10.58 to 6.84 ng/g), respectively, which effectively reduced the allergenicity of crab paste. Consequently, ACP treatment successfully suppressed microbial proliferation in crab paste and potentially altered the composition of the microbial population, as well as impacted functional prediction metabolic pathways. This study demonstrated the significant potential of ACP treatment in controlling microbial populations in crab products.</p>

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Effect of atmospheric cold plasma treatment on microbial diversity, myofibrillar protein oxidation, and allergenic protein contents in refrigerated crab paste (Portunus trituberculatus)

  • 期刊论文

  • 发表日期：

    2025年10月3日

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  • Qinyun Jiang,
  • Yanwei Liu,
  • Siwei Peng,
  • Yingyun Chen,
  • Liuying Li,
  • Jing Chen,
  • Bin Zhang,
  • Shanggui Deng

摘要

This study aimed to investigate the effects of Atmospheric cold plasma (ACP) treatment on microbial communities and ACP-induced oxidation on proteins in crab paste during 6-day storage at 4 °C. 16 S ribosomal RNA (16 S rRNA) gene amplicon sequencing was used to analyze changes in the bacterial community of the crab paste through indices including bacterial composition, alpha diversity, beta diversity, and Linear discriminant analysis Effect Size (LEfSe) analysis, while protein changes were assessed by examining the secondary structure and allergenic protein content. The results demonstrated that ACP-treated samples exhibited significantly reduced microbial indices compared to the control group. Vibrio emerged as the predominant species in both groups during the storage. Compared with the control group, ACP treatment eliminated most bacterial genera, including Variovorax, Vibrionimonas, Bradyrhizobium, Burkholderia-Caballeronia-Paraburkholderia, Hydrogenophaga, and Pseudomonas. Functional prediction analysis indicated that ACP treatment and storage conditions substantially affected metabolic pathways, especially those associated with carbohydrate, amino acid, and energy metabolism. Besides, oxidation induced by ACP treatment- led to substantial alterations in the secondary structure of myofibrillar protein. ACP treatment increased random coil content by 83.33%, while reducing α-helix, β-turn, and β-sheet by 4.88%, 14.29%, and 21.05%, respectively. The content of allergenic proteins, like tropomyosin (TM) and arginine kinase (AK), decreased by 24.31% (from 38.54 to 29.17 ng/g) and 35.35% (from 10.58 to 6.84 ng/g), respectively, which effectively reduced the allergenicity of crab paste. Consequently, ACP treatment successfully suppressed microbial proliferation in crab paste and potentially altered the composition of the microbial population, as well as impacted functional prediction metabolic pathways. This study demonstrated the significant potential of ACP treatment in controlling microbial populations in crab products.