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<p>Apple pomace (AP) holds significant nutritional and health-promoting potential due to its dietary fiber (DF) content. However, integrating AP into food formulations requires addressing its highly insoluble nature, which limits its functionality. This study investigates the efficacy of enzymatic treatment as a sustainable and cost-effective approach to modify the insoluble fraction and enhance DF techno-functional properties. The effects of a carbohydrase complex on AP functionality and its soluble uronic acid (SUA) and soluble neutral sugar (SNS) contents were assessed by optimizing enzyme concentration (0.5–5 mL/kg dry weight (DW)), hydrolysis time (1–4&#xa0;h), and temperature (40–60&#xa0;°C) using response-surface methodology with a central composite design. The optimization process identified the ideal conditions to be 3.31&#xa0;h of hydrolysis at an enzyme concentration of 4.1 mL/kg DW and a temperature of 48&#xa0;°C, which significantly improved treatment efficiency and maximized all evaluated responses simultaneously. Under these conditions, the solubility of AP, soluble uronic acid content and soluble neutral sugar content increased by 1.70, 3.60 and 2.48 times, respectively, compared to untreated AP. The water and oil retention capacities decreased by 16% and 15%. Furthermore, a 35% increase in soluble DF and a 39% decrease in insoluble DF were observed under optimal conditions. This research highlights the potential of optimizing enzymatic processing to transform fruit by-products into novel food ingredients with enhanced solubility.</p>

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Optimizing enzymatic processing of apple pomace: a strategy for modifying techno-functional properties and dietary fiber

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年9月27日

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  • Alba Díaz-Núñez,
  • Gloria López-Gámez,
  • Olga Martín-Belloso,
  • Robert Soliva-Fortuny,
  • Pedro Elez-Martínez

摘要

Apple pomace (AP) holds significant nutritional and health-promoting potential due to its dietary fiber (DF) content. However, integrating AP into food formulations requires addressing its highly insoluble nature, which limits its functionality. This study investigates the efficacy of enzymatic treatment as a sustainable and cost-effective approach to modify the insoluble fraction and enhance DF techno-functional properties. The effects of a carbohydrase complex on AP functionality and its soluble uronic acid (SUA) and soluble neutral sugar (SNS) contents were assessed by optimizing enzyme concentration (0.5–5 mL/kg dry weight (DW)), hydrolysis time (1–4 h), and temperature (40–60 °C) using response-surface methodology with a central composite design. The optimization process identified the ideal conditions to be 3.31 h of hydrolysis at an enzyme concentration of 4.1 mL/kg DW and a temperature of 48 °C, which significantly improved treatment efficiency and maximized all evaluated responses simultaneously. Under these conditions, the solubility of AP, soluble uronic acid content and soluble neutral sugar content increased by 1.70, 3.60 and 2.48 times, respectively, compared to untreated AP. The water and oil retention capacities decreased by 16% and 15%. Furthermore, a 35% increase in soluble DF and a 39% decrease in insoluble DF were observed under optimal conditions. This research highlights the potential of optimizing enzymatic processing to transform fruit by-products into novel food ingredients with enhanced solubility.