Scholarlink

<p>This study examines the metabolomic profiles and bioactive properties of raw and germinated cluster bean (<i>Cyamopsis tetragonoloba</i>) seeds, focusing on their α-amylase inhibitory activity. A key element of diabetes management is inhibition of α-amylase, which delays the breakdown of starch into glucose and lowers postprandial blood glucose spikes. Plant-derived α-amylase inhibitors have health benefits and are safer than manufactured medications that have adverse effects from prolonged use. Metabolomic analysis revealed 108 compounds in raw seeds and 124 in germinated seeds, with 18 shared compounds showing high similarity (&gt; 70%). These compounds include phytochemicals, amino acids, peptides, lipids, and carbohydrates. Raw seeds displayed higher total phenolic content, and antioxidant activity (DPPH: 82.91 ± 0.13%, ABTS: 85.56 ± 1.02%, FRAP: 62.50 ± 0.00&#xa0;mg/g ascorbic acid equivalents) compared to germinated seeds. However, germination significantly increased α-amylase inhibitory activity (IC<sub>50</sub>: 0.75&#xa0;mg/mL vs. 7.00&#xa0;mg/mL in raw seeds). Furthermore, peptides revealed from metabolomics profile and exhibiting peptide ranking scores &gt; 0.5 were evaluated using ADMET and molecular docking studies. From the evaluated peptides, maculosin noted from germinated seeds exhibited strong binding affinity (-7.4&#xa0;kcal/mol) to human pancreatic α-amylase, comparable to acarbose (-7.6&#xa0;kcal/mol), and high human intestinal absorption. These results highlight germinated cluster bean seeds as a rich source of natural α-amylase inhibitors, with potential applications in diabetes management.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Germinated cluster bean seeds: enhanced α-amylase inhibition and metabolomic insights for diabetes management

  • 期刊论文

  • 发表日期：

    2025年7月22日

查看全文
  • Komal Solanki,
  • Hitarth Kansara,
  • Vishwam Singh,
  • Stuti Pandya,
  • Krutika Abhyankar

摘要

This study examines the metabolomic profiles and bioactive properties of raw and germinated cluster bean (Cyamopsis tetragonoloba) seeds, focusing on their α-amylase inhibitory activity. A key element of diabetes management is inhibition of α-amylase, which delays the breakdown of starch into glucose and lowers postprandial blood glucose spikes. Plant-derived α-amylase inhibitors have health benefits and are safer than manufactured medications that have adverse effects from prolonged use. Metabolomic analysis revealed 108 compounds in raw seeds and 124 in germinated seeds, with 18 shared compounds showing high similarity (> 70%). These compounds include phytochemicals, amino acids, peptides, lipids, and carbohydrates. Raw seeds displayed higher total phenolic content, and antioxidant activity (DPPH: 82.91 ± 0.13%, ABTS: 85.56 ± 1.02%, FRAP: 62.50 ± 0.00 mg/g ascorbic acid equivalents) compared to germinated seeds. However, germination significantly increased α-amylase inhibitory activity (IC50: 0.75 mg/mL vs. 7.00 mg/mL in raw seeds). Furthermore, peptides revealed from metabolomics profile and exhibiting peptide ranking scores > 0.5 were evaluated using ADMET and molecular docking studies. From the evaluated peptides, maculosin noted from germinated seeds exhibited strong binding affinity (-7.4 kcal/mol) to human pancreatic α-amylase, comparable to acarbose (-7.6 kcal/mol), and high human intestinal absorption. These results highlight germinated cluster bean seeds as a rich source of natural α-amylase inhibitors, with potential applications in diabetes management.