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<p>This study evaluated the efficacy of gaseous allyl isothiocyanate (AITC) in inhibiting <i>Aspergillus parasiticus</i> growth and aflatoxin production in soybeans and beans stored at 85% relative humidity for 35d. Samples of 50&#xa0;g of soybeans or beans were inoculated with <i>A. parasiticus</i> (10⁴ spores/g) and treated with 0, 1, 5, or 25 µL/L of gaseous AITC in hermetically sealed glass jars. After 35d, microbial counts were determined on potato dextrose agar, and aflatoxins were extracted, concentrated using immunoaffinity columns, and quantified via high-performance liquid chromatography with fluorescence detection. The moisture content of the grains was also monitored throughout the storage period. The results showed that AITC at 5 and 25 µL/L reduced <i>A. parasiticus</i> by 2 and 4 Log CFU/g in soybeans, respectively. In beans, reductions of 2 and 5 Log CFU/g were observed at the same concentrations. Aflatoxin B1 (AFB1) production was completely inhibited at 25 µL/L and reduced by approximately 90% at 5 µL/L. Gaseous AITC at 1 µL/L was ineffective in inhibiting fungal growth in soybeans and did not reduce AFB1 production in either crop. During storage, the moisture in soybeans increased from 14 to 18%, while the moisture in beans rose from 14.5 to 19%. These findings suggest that AITC fumigation may be a viable strategy to prevent <i>A. parasiticus</i> growth and AFB1 contamination in stored soybeans and beans, potentially improving food safety and grain preservation.</p>

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Allyl isothiocyanate as a natural fumigant for protecting soybean and bean grains against Aspergillus parasiticus growth and aflatoxin production

  • 期刊论文

  • 发表日期：

    2025年5月28日

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  • Lucas Ferreira Lopes,
  • Vitor Rampazzo Favoretto,
  • Alberto Gonçalves Evangelista,
  • Keliani Bordin,
  • Claudemir Zucareli,
  • Fernando Bittencourt Luciano

摘要

This study evaluated the efficacy of gaseous allyl isothiocyanate (AITC) in inhibiting Aspergillus parasiticus growth and aflatoxin production in soybeans and beans stored at 85% relative humidity for 35d. Samples of 50 g of soybeans or beans were inoculated with A. parasiticus (10⁴ spores/g) and treated with 0, 1, 5, or 25 µL/L of gaseous AITC in hermetically sealed glass jars. After 35d, microbial counts were determined on potato dextrose agar, and aflatoxins were extracted, concentrated using immunoaffinity columns, and quantified via high-performance liquid chromatography with fluorescence detection. The moisture content of the grains was also monitored throughout the storage period. The results showed that AITC at 5 and 25 µL/L reduced A. parasiticus by 2 and 4 Log CFU/g in soybeans, respectively. In beans, reductions of 2 and 5 Log CFU/g were observed at the same concentrations. Aflatoxin B1 (AFB1) production was completely inhibited at 25 µL/L and reduced by approximately 90% at 5 µL/L. Gaseous AITC at 1 µL/L was ineffective in inhibiting fungal growth in soybeans and did not reduce AFB1 production in either crop. During storage, the moisture in soybeans increased from 14 to 18%, while the moisture in beans rose from 14.5 to 19%. These findings suggest that AITC fumigation may be a viable strategy to prevent A. parasiticus growth and AFB1 contamination in stored soybeans and beans, potentially improving food safety and grain preservation.