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<p>This study developed three structurally modified derivatives (acetylated rEPS-2&#xa0;A, phosphorylated rEPS-2P, and carboxymethylated rEPS-2&#xa0;C) from rEPS-2, a bioactive exopolysaccharide produced by <i>Bacillus velezensis</i> SN-1. Comprehensive characterization using gel permeation chromatography, FT-IR, and NMR spectroscopy confirmed successful functional group incorporation, while SEM and TGA analyses revealed significant alterations in surface morphology and thermal stability. Biological evaluation demonstrated that carboxymethylated rEPS-2&#xa0;C exhibited markedly enhanced antioxidant capacity (showing 28.6% increase in DPPH scavenging activity compared to native rEPS-2) and superior antitumor effects (with 75.35% inhibition against HepG-2 cells), outperforming other derivatives. These findings highlight the structure-activity relationship of polysaccharide modifications and position rEPS-2&#xa0;C as a promising dual-functional agent for oxidative stress-related disorders and cancer therapy applications.</p>

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Characterization and chemical modification of a Bacillus velezensis SN-1 exopolysaccharide, rEPS-2

  • 期刊论文

  • 发表日期：

    2025年5月29日

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  • Rina Wu,
  • Yao Liu,
  • Yuzhe Gao,
  • Xiaofei Yang,
  • Qingyu Yang,
  • Chengxu Cao

摘要

This study developed three structurally modified derivatives (acetylated rEPS-2 A, phosphorylated rEPS-2P, and carboxymethylated rEPS-2 C) from rEPS-2, a bioactive exopolysaccharide produced by Bacillus velezensis SN-1. Comprehensive characterization using gel permeation chromatography, FT-IR, and NMR spectroscopy confirmed successful functional group incorporation, while SEM and TGA analyses revealed significant alterations in surface morphology and thermal stability. Biological evaluation demonstrated that carboxymethylated rEPS-2 C exhibited markedly enhanced antioxidant capacity (showing 28.6% increase in DPPH scavenging activity compared to native rEPS-2) and superior antitumor effects (with 75.35% inhibition against HepG-2 cells), outperforming other derivatives. These findings highlight the structure-activity relationship of polysaccharide modifications and position rEPS-2 C as a promising dual-functional agent for oxidative stress-related disorders and cancer therapy applications.