Scholarlink

<p>The establishment of monoclonal, stably transduced cell lines is a critical step in functional genomics and drug discovery. However, conventional methods are often time-consuming, labor-intensive, and prone to compromising cell viability. Here, we present a microfluidic single-cell sorting system based on laser-induced jetting (LIJet) that significantly improves the efficiency and quality of stable cell line generation. This system integrates a light-responsive substrate with metal coating and a PDMS microfluidic chip featuring an array of microwells, enabling single-cell capture, identification, and non-contact precision release. A 532&#xa0;nm nanosecond pulsed laser is used to generate localized microjets, which accurately eject target cells from the microwells. In addition to achieving a 100% sorting success rate and maintaining over 95.3% post-sorting cell viability, the system supports long-term on-chip culture and viral transduction with full real-time monitoring. We demonstrated the platform’s functionality by performing on-chip ZsGreen lentiviral transduction of human lung adenocarcinoma PC9 cells, followed by fluorescence-based single-cell selection, ultimately establishing monoclonal cell lines with stable transgene expression. This platform offers notable advantages in low-damage manipulation, dynamic monitoring, and functional perturbation, providing a robust and efficient solution for the construction of stably transduced cell lines, gene function screening, and phenotypic analysis across a variety of biomedical applications.</p> Graphical abstract <p></p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

High-efficiency generation of stably transduced monoclonal cell lines via a laser-induced jetting microfluidic platform

  • 期刊论文

  • 发表日期：

    2026年5月1日

查看全文
  • Fuyuan Chen,
  • Baojian Zhang,
  • Yuntong Wang,
  • Hao Peng,
  • Yu Wang,
  • Zhixiong Song,
  • Meina Chen,
  • Xuan Yang,
  • Peng Liang,
  • Yue Qu,
  • Bei Li

摘要

The establishment of monoclonal, stably transduced cell lines is a critical step in functional genomics and drug discovery. However, conventional methods are often time-consuming, labor-intensive, and prone to compromising cell viability. Here, we present a microfluidic single-cell sorting system based on laser-induced jetting (LIJet) that significantly improves the efficiency and quality of stable cell line generation. This system integrates a light-responsive substrate with metal coating and a PDMS microfluidic chip featuring an array of microwells, enabling single-cell capture, identification, and non-contact precision release. A 532 nm nanosecond pulsed laser is used to generate localized microjets, which accurately eject target cells from the microwells. In addition to achieving a 100% sorting success rate and maintaining over 95.3% post-sorting cell viability, the system supports long-term on-chip culture and viral transduction with full real-time monitoring. We demonstrated the platform’s functionality by performing on-chip ZsGreen lentiviral transduction of human lung adenocarcinoma PC9 cells, followed by fluorescence-based single-cell selection, ultimately establishing monoclonal cell lines with stable transgene expression. This platform offers notable advantages in low-damage manipulation, dynamic monitoring, and functional perturbation, providing a robust and efficient solution for the construction of stably transduced cell lines, gene function screening, and phenotypic analysis across a variety of biomedical applications.

Graphical abstract