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<p>Zearalenone (ZEN) is a mycotoxin widely present in crops, posing a serious threat to human health. Therefore, it is of great significance to establish a rapid and accurate method for zearalenone detection. In this work, a microfluidic device was produced using 3D printing technology and employed to construct a high-throughput fluorescence analysis platform. The blue fluorescent carbon quantum dots (B-CDs) and green fluorescent carbon quantum dots (G-CDs) were synthesized and incorporated into separate detection areas to achieve different signal responses. The B-CDs-cDNA/Apt1 and G-CDs-Apt2 complexes are pre-positioned in this area. The two-dimensional layered nanomaterial Zn-TCPP is used as an efficient quencher, achieving specific quenching by its adsorption on single-stranded DNA. Finally, high-throughput rapid detection was achieved by identifying RGB values via a smartphone. Under optimal conditions, the detection range of the fluorescent aptasensor is 0.5–500&#xa0;ng/mL, with a limit of detection of 1.325&#xa0;pg/mL. Moreover, this high-throughput fluorescent aptasensor demonstrates excellent analytical performance in the detection of real samples. This work provides a novel strategy for the rapid detection of zearalenone.</p> Graphical Abstract <p></p>

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High-throughput microfluidic fluorescent aptasensor platform integrating dual carbon dots and Zn-TCPP for rapid detection of zearalenone

  • 期刊论文

  • 发表日期：

    2026年3月19日

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  • Yanjun Yin,
  • Hailin Shen,
  • Jiayi Sun,
  • Zhiguang Suo

摘要

Zearalenone (ZEN) is a mycotoxin widely present in crops, posing a serious threat to human health. Therefore, it is of great significance to establish a rapid and accurate method for zearalenone detection. In this work, a microfluidic device was produced using 3D printing technology and employed to construct a high-throughput fluorescence analysis platform. The blue fluorescent carbon quantum dots (B-CDs) and green fluorescent carbon quantum dots (G-CDs) were synthesized and incorporated into separate detection areas to achieve different signal responses. The B-CDs-cDNA/Apt1 and G-CDs-Apt2 complexes are pre-positioned in this area. The two-dimensional layered nanomaterial Zn-TCPP is used as an efficient quencher, achieving specific quenching by its adsorption on single-stranded DNA. Finally, high-throughput rapid detection was achieved by identifying RGB values via a smartphone. Under optimal conditions, the detection range of the fluorescent aptasensor is 0.5–500 ng/mL, with a limit of detection of 1.325 pg/mL. Moreover, this high-throughput fluorescent aptasensor demonstrates excellent analytical performance in the detection of real samples. This work provides a novel strategy for the rapid detection of zearalenone.

Graphical Abstract