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<p>Capillary electrophoresis (CE) is primarily a technique for separating and analyzing samples based on their differing physicochemical properties. CE is renowned for its rapid analytical capability and minimal sample requirements, rendering it widely applicable in the biomedical field. However, the silica hydroxyl groups (Si–OH) present on the capillary wall can adversely affect protein separation. Modifying capillary walls through physical adsorption or chemical bonding can suppress protein adsorption, thereby enhancing separation efficiency. This review examines the causes and solutions for protein adsorption, types of capillary coatings, and the application of coated capillaries in protein separation. Finally, it outlines future trends for coated capillaries in protein separation.</p> Graphical Abstract <p></p>

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Research advances in capillary electrophoresis coating technology for protein analysis

  • 期刊论文

  • 发表日期：

    2025年12月18日

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  • Mengyuan Xu,
  • Wei Pan,
  • Yichen Tian,
  • Hailin Cong,
  • Bing Yu

摘要

Capillary electrophoresis (CE) is primarily a technique for separating and analyzing samples based on their differing physicochemical properties. CE is renowned for its rapid analytical capability and minimal sample requirements, rendering it widely applicable in the biomedical field. However, the silica hydroxyl groups (Si–OH) present on the capillary wall can adversely affect protein separation. Modifying capillary walls through physical adsorption or chemical bonding can suppress protein adsorption, thereby enhancing separation efficiency. This review examines the causes and solutions for protein adsorption, types of capillary coatings, and the application of coated capillaries in protein separation. Finally, it outlines future trends for coated capillaries in protein separation.

Graphical Abstract