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<p>The timely and cost-effective detection of infectious pathogens is critical for their prevention and control. In this study, a low-cost, portable, and user-friendly WiFi-enabled Internet of Things (IoT) monitoring system was developed for the detection of infectious pathogens. The detection mechanism relies on real-time monitoring of hydroxy naphthol blue (HNB) colorimetric signals, which are triggered by the depletion of Mg<sup>2+</sup> ions during loop-mediated isothermal amplification (LAMP). A 3D-printed, portable reader was developed for both LAMP incubation and real-time HNB signal monitoring. Analysis at an incubation temperature of 65&#xa0;°C demonstrated the excellent thermal stability of HNB and its linear correlation (<i>R</i><sup>2</sup> = 0.9973) with Mg<sup>2+</sup> ion consumption, highlighting its suitability as a real-time indicator. The detection capability was validated using&#xa0;the <i>Nosema bombycis</i> SSU rRNA gene and&#xa0;the <i>Bombyx mori</i> nucleopolyhedrovirus <i>gp41</i> gene as model targets. The results showed linear relationships (<i>R</i><sup>2</sup> &gt; 0.99) between threshold time and log target concentration, with the limit of detection (LOD) reaching 1 copy µL<sup>−1</sup> for both targets. Analysis of <i>Nosema bombycis</i> in real samples showed comparable performance to the conventional female moth microscopy method. Furthermore, the IoT-enabled reader allowed for fully automated result reporting via email, facilitating timely implementation of containment measures. This study reported on the development of a portable and user-friendly IoT-based test platform, presenting attractive potential for point-of-care diagnosis in infectious diseases, particularly in resource-constrained settings.</p> Graphical Abstract <p></p>

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IoT-integrated and portable real-time LAMP platform with hydroxy naphthol blue (HNB) for universal, low-cost pathogen detection in resource-limited settings

  • 期刊论文

  • 发表日期：

    2025年12月19日

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  • Haojie Zhao,
  • Fei Han,
  • Xinyue Sun,
  • Zexi Gao,
  • Zeyan Zheng,
  • Yajing Niu,
  • Wenping Li,
  • Chao Su,
  • Zhonghong Li,
  • Wenzhi Tang

摘要

The timely and cost-effective detection of infectious pathogens is critical for their prevention and control. In this study, a low-cost, portable, and user-friendly WiFi-enabled Internet of Things (IoT) monitoring system was developed for the detection of infectious pathogens. The detection mechanism relies on real-time monitoring of hydroxy naphthol blue (HNB) colorimetric signals, which are triggered by the depletion of Mg2+ ions during loop-mediated isothermal amplification (LAMP). A 3D-printed, portable reader was developed for both LAMP incubation and real-time HNB signal monitoring. Analysis at an incubation temperature of 65 °C demonstrated the excellent thermal stability of HNB and its linear correlation (R2 = 0.9973) with Mg2+ ion consumption, highlighting its suitability as a real-time indicator. The detection capability was validated using the Nosema bombycis SSU rRNA gene and the Bombyx mori nucleopolyhedrovirus gp41 gene as model targets. The results showed linear relationships (R2 > 0.99) between threshold time and log target concentration, with the limit of detection (LOD) reaching 1 copy µL−1 for both targets. Analysis of Nosema bombycis in real samples showed comparable performance to the conventional female moth microscopy method. Furthermore, the IoT-enabled reader allowed for fully automated result reporting via email, facilitating timely implementation of containment measures. This study reported on the development of a portable and user-friendly IoT-based test platform, presenting attractive potential for point-of-care diagnosis in infectious diseases, particularly in resource-constrained settings.

Graphical Abstract