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<p>Human epidermal growth factor receptor 2 (HER2) is a key biomarker associated with breast cancer, and its reliable detection using effective diagnostic tools is clinically significant. Here, we developed an electrochemical aptasensor based on an electroactive labeling strategy for the detection of HER2 using differential pulse voltammetry (DPV). A nickel nanofoam/gold nanoparticles (AuNPs)/silver nanoparticles (AgNPs) nanocomposite was employed as a novel electrode modifier synthesized via a simple, low-cost, and reproducible method. In this design, AuNPs enabled stable aptamer immobilization, while AgNPs acted as electroactive labels, enhancing the current response through a synergistic effect that could not be achieved with either nanoparticle alone. The newly developed aptasensor demonstrated high sensitivity and good reproducibility for HER2 detection. The aptasensor showed a wide linear range of 0.001–100&#xa0;ng&#xa0;mL<sup>−1</sup>, a very low limit of detection (LOD) of 0.30&#xa0;pg&#xa0;mL<sup>−1</sup>, and a limit of quantification (LOQ) of 1.0&#xa0;pg&#xa0;mL<sup>−1</sup>, indicating its significant potential for clinical diagnostic applications.</p>

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Electrochemical aptasensing of HER2 breast cancer biomarker using a Ni nanofoam/Ag–Au ternary nanocomposite

  • 期刊论文

  • 发表日期：

    2025年12月18日

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  • Farzaneh Hoseynidokht,
  • Mohammad Mazloum-Ardakani,
  • Fatemeh Hakimian,
  • Nafiseh Sahraei

摘要

Human epidermal growth factor receptor 2 (HER2) is a key biomarker associated with breast cancer, and its reliable detection using effective diagnostic tools is clinically significant. Here, we developed an electrochemical aptasensor based on an electroactive labeling strategy for the detection of HER2 using differential pulse voltammetry (DPV). A nickel nanofoam/gold nanoparticles (AuNPs)/silver nanoparticles (AgNPs) nanocomposite was employed as a novel electrode modifier synthesized via a simple, low-cost, and reproducible method. In this design, AuNPs enabled stable aptamer immobilization, while AgNPs acted as electroactive labels, enhancing the current response through a synergistic effect that could not be achieved with either nanoparticle alone. The newly developed aptasensor demonstrated high sensitivity and good reproducibility for HER2 detection. The aptasensor showed a wide linear range of 0.001–100 ng mL−1, a very low limit of detection (LOD) of 0.30 pg mL−1, and a limit of quantification (LOQ) of 1.0 pg mL−1, indicating its significant potential for clinical diagnostic applications.