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<p>This study developed a novel biosensor for <i>Vibrio parahaemolyticus</i> detection by integrating DNA hydrogel with a portable glucose meter. The sensor employs aptamer-functionalized DNA hydrogel as the recognition element, achieving signal transduction through a target-responsive hydrogel dissociation mechanism. The presence of <i>Vibrio parahaemolyticus</i> initiates aptamer binding, destabilizes the DNA hydrogel network, and releases preloaded invertase. This enzyme converts sucrose into glucose, enabling quantitative detection using a portable glucose meter. The sensor demonstrated exceptional performance, achieving a broad dynamic range (10<sup>1</sup>–10<sup>5</sup>&#xa0;CFU/mL) for <i>Vibrio parahaemolyticus</i> detection with a detection limit of 6&#xa0;CFU/mL, representing a significant improvement over existing methods. This research provides a promising solution for point-of-care testing of foodborne pathogens, with substantial application potential in food safety monitoring and clinical diagnostics.</p> Graphical abstract <p></p>

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Biosensor of Vibrio parahaemolyticus based on DNA hydrogel–encapsulated invertase and blood glucose meter

  • 期刊论文

  • 发表日期：

    2025年11月14日

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  • Bingyan Liu,
  • Hongyu Zhou,
  • Yao Xu,
  • Shengjun Bu,
  • Zhuo Hao,
  • Songlei Guan,
  • Jiayu Wan

摘要

This study developed a novel biosensor for Vibrio parahaemolyticus detection by integrating DNA hydrogel with a portable glucose meter. The sensor employs aptamer-functionalized DNA hydrogel as the recognition element, achieving signal transduction through a target-responsive hydrogel dissociation mechanism. The presence of Vibrio parahaemolyticus initiates aptamer binding, destabilizes the DNA hydrogel network, and releases preloaded invertase. This enzyme converts sucrose into glucose, enabling quantitative detection using a portable glucose meter. The sensor demonstrated exceptional performance, achieving a broad dynamic range (101–105 CFU/mL) for Vibrio parahaemolyticus detection with a detection limit of 6 CFU/mL, representing a significant improvement over existing methods. This research provides a promising solution for point-of-care testing of foodborne pathogens, with substantial application potential in food safety monitoring and clinical diagnostics.

Graphical abstract