Scholarlink

<p>Interleukin-6 (IL-6) is an acute phase response protein. Its level rapidly increases in infection, trauma, and acute inflammatory states. It usually appears earlier than other inflammatory markers such as C-reactive protein and procalcitonin. Therefore, detecting abnormal IL-6 levels (The clinical reference range is greater than 7&#xa0;pg&#xa0;mL<sup>−1</sup>) faster can help humans receive timely treatment and recover their health. This article successfully synthesized a medium-entropy oxide nanoenzyme. It does not require the involvement of hydrogen peroxide and has a low Michaelis constant of 0.101&#xa0;mM. Based on the interaction between La-based medium-entropy oxide (MEO-La) nanoenzyme and secondary antibody, specific detection of IL-6 in enzyme-linked immunosorbent assay is achieved. The concentration of IL-6 in human serum can be qualitatively detected. The detection limit and linear range are 0.03&#xa0;pg&#xa0;mL<sup>−1</sup> and 5–1000&#xa0;pg&#xa0;mL<sup>−1</sup>, respectively. This work not only is of great significance for better understanding the unique properties of medium-entropy nanoenzymes but also has enormous potential for biochemical sensing of human health.</p> Graphical Abstract <p>This work develops a medium entropy oxide nanoenzyme for ultrasensitive IL-6 detection, achieving a detection limit of 0.03&#xa0;pg&#xa0;mL<sup>−1</sup>. The hydrogen peroxide free system has shown great potential in early disease diagnosis and biochemical sensing applications.</p> <p></p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Medium-entropy nanoenzyme with high oxidase-like activity for sensitive detection of interleukin-6 based on ELISA

  • 期刊论文

  • 发表日期：

    2025年8月26日

查看全文
  • Lifang Chen,
  • Jiaqi Chen,
  • Ying Lei,
  • Pengcheng Lin,
  • Donglin Cao,
  • Wei Xiao,
  • Liangshan Hu

摘要

Interleukin-6 (IL-6) is an acute phase response protein. Its level rapidly increases in infection, trauma, and acute inflammatory states. It usually appears earlier than other inflammatory markers such as C-reactive protein and procalcitonin. Therefore, detecting abnormal IL-6 levels (The clinical reference range is greater than 7 pg mL−1) faster can help humans receive timely treatment and recover their health. This article successfully synthesized a medium-entropy oxide nanoenzyme. It does not require the involvement of hydrogen peroxide and has a low Michaelis constant of 0.101 mM. Based on the interaction between La-based medium-entropy oxide (MEO-La) nanoenzyme and secondary antibody, specific detection of IL-6 in enzyme-linked immunosorbent assay is achieved. The concentration of IL-6 in human serum can be qualitatively detected. The detection limit and linear range are 0.03 pg mL−1 and 5–1000 pg mL−1, respectively. This work not only is of great significance for better understanding the unique properties of medium-entropy nanoenzymes but also has enormous potential for biochemical sensing of human health.

Graphical Abstract

This work develops a medium entropy oxide nanoenzyme for ultrasensitive IL-6 detection, achieving a detection limit of 0.03 pg mL−1. The hydrogen peroxide free system has shown great potential in early disease diagnosis and biochemical sensing applications.