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<p>Non-invasive glucose sensing technology exhibits significant clinical potential for continuous diabetes monitoring and early screening in high-risk populations. Herein, we developed a modular fluorescent biosensor for salivary glucose monitoring through synergistic integration of a detection module (glucose oxidase) and a signal transduction module (PL DNAzyme-substrate complex). The sensing system employs a fluorophore/quencher pair-modified PL DNAzyme-substrate complex as a fluorescence switching element. Experimental results showed a linear detection range from 40&#xa0;nM to 1.0&#xa0;mM with a detection limit of 4.3&#xa0;nM, demonstrating a 1163-fold improvement in sensitivity compared with the previous version. The biosensor exhibited excellent specificity and stability. It was used to detect 2-h postprandial saliva samples from 15 participants (mean glucose concentration: 53.7 ± 19.5&#xa0;μM), and its extended functionality was demonstrated through continuous daily monitoring of three participants and 14-day consecutive tracking tests, which successfully captured temporal glucose fluctuations. This work presents a fluorescence-based platform for non-invasive salivary glucose monitoring, while its modular architecture provides a versatile framework for future performance optimization through component substitution.</p> Graphical Abstract <p></p>

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A fluorescent biosensor based on glucose oxidase-DNAzyme/substrate complex synergy for salivary glucose monitoring

  • 期刊论文

  • 发表日期：

    2025年8月9日

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  • Longping Yang,
  • Huiyu Tian,
  • Chengcheng Liu,
  • Fangning Jiang,
  • Yan Dong,
  • Yongjie Sheng,
  • Yanhong Sun,
  • Yanqun Fei,
  • Jiacui Xu,
  • Dazhi Jiang

摘要

Non-invasive glucose sensing technology exhibits significant clinical potential for continuous diabetes monitoring and early screening in high-risk populations. Herein, we developed a modular fluorescent biosensor for salivary glucose monitoring through synergistic integration of a detection module (glucose oxidase) and a signal transduction module (PL DNAzyme-substrate complex). The sensing system employs a fluorophore/quencher pair-modified PL DNAzyme-substrate complex as a fluorescence switching element. Experimental results showed a linear detection range from 40 nM to 1.0 mM with a detection limit of 4.3 nM, demonstrating a 1163-fold improvement in sensitivity compared with the previous version. The biosensor exhibited excellent specificity and stability. It was used to detect 2-h postprandial saliva samples from 15 participants (mean glucose concentration: 53.7 ± 19.5 μM), and its extended functionality was demonstrated through continuous daily monitoring of three participants and 14-day consecutive tracking tests, which successfully captured temporal glucose fluctuations. This work presents a fluorescence-based platform for non-invasive salivary glucose monitoring, while its modular architecture provides a versatile framework for future performance optimization through component substitution.

Graphical Abstract