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<p>Preschool-aged children and non-pregnant women of reproductive age particularly in countries of a lower socio-demographic index may experience insufficient dietary vitamin A. Fortifying staple foods such as edible oils with vitamin A is a useful approach to reduce malnutrition. To enable better quality oil fortification, producers need reliable and inexpensive tools for assessment of the vitamin A contents of their products. A simple reversed-phase high-performance thin-layer chromatography fluorescence detection (RP–HPTLC–FLD) method was developed and studied for the quantification of vitamin A palmitate (VAP) in five edible plant oils. The challenging matrices of non-refined palm kernel oil and soybean oil, which are known to interfere with the accuracy of some easy-to-use test kits, were separated&#xa0;from VAP using the new RP–HPTLC–FLD method. The standard addition method provided good accuracy and acceptable calibration curves (coefficients of determination R<sup>2</sup> between 0.9874 and 0.9999) even for manual application. The developed RP–HPTLC–FLD method proved simple, robust, sustainable, cost-efficient, and suitable for low-tech (minimum equipment) on-site analysis.&#xa0;</p> Graphical Abstract <p></p>

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Proposed RP–HPTLC–FLD kit for analysis of vitamin A palmitate in edible oils

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年5月19日

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  • Daniel Meyer,
  • Sophia Hörnlein,
  • Dietrich Rein,
  • Gertrud E. Morlock

摘要

Preschool-aged children and non-pregnant women of reproductive age particularly in countries of a lower socio-demographic index may experience insufficient dietary vitamin A. Fortifying staple foods such as edible oils with vitamin A is a useful approach to reduce malnutrition. To enable better quality oil fortification, producers need reliable and inexpensive tools for assessment of the vitamin A contents of their products. A simple reversed-phase high-performance thin-layer chromatography fluorescence detection (RP–HPTLC–FLD) method was developed and studied for the quantification of vitamin A palmitate (VAP) in five edible plant oils. The challenging matrices of non-refined palm kernel oil and soybean oil, which are known to interfere with the accuracy of some easy-to-use test kits, were separated from VAP using the new RP–HPTLC–FLD method. The standard addition method provided good accuracy and acceptable calibration curves (coefficients of determination R2 between 0.9874 and 0.9999) even for manual application. The developed RP–HPTLC–FLD method proved simple, robust, sustainable, cost-efficient, and suitable for low-tech (minimum equipment) on-site analysis. 

Graphical Abstract