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<p>Metal-organic frameworks (MOFs) are&#xa0;often applied for enzyme immobilization, while they are limited for bioelectrochemical applications due to poor electronic conductivity. Two-dimensional (2D) metal-organic layers (MOLs) with an ultra-thin lamellar structure can effectively shorten the electron transport path and improve the electron transfer rate. In this study, ferrocene as an electron mediator is covalently bound to a 2D-MOL (Fc-NH<sub>2</sub>-Hf-BTB-MOL) to accelerate electron transfer&#xa0;between the electrode surface and enzyme. Glucose oxidase (GOx) is immobilized on&#xa0;the electrode modified with Fc-NH<sub>2</sub>-Hf-BTB-MOL with the addition of chitosan and carboxylated carbon nanotubes. Electrochemical tests such as cyclic voltammetry are carried out&#xa0;on the glucose biosensor, which shows&#xa0;linear detection ranges of 5 ~ 400&#xa0;μM and 3 ~ 9&#xa0;mM, with a detection limit of 3.9&#xa0;μM (S/N = 3). Therefore, this strategy of construction of an enzyme electrode based on 2D-MOLs with&#xa0;enhanced electron transfer results in a biosensor with excellent specificity and activity for practical glucose detection.</p>

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Enzymatic bioelectrodes based on ferrocene-modified metal-organic layers for electrochemical glucose detection

  • 期刊论文

  • 发表日期：

    2025年2月27日

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  • Lingling Dong,
  • Xuefu Zeng,
  • Yu Xiong,
  • Xinxin Xiao,
  • Dongping Zhan,
  • Shizhen Wang

摘要

Metal-organic frameworks (MOFs) are often applied for enzyme immobilization, while they are limited for bioelectrochemical applications due to poor electronic conductivity. Two-dimensional (2D) metal-organic layers (MOLs) with an ultra-thin lamellar structure can effectively shorten the electron transport path and improve the electron transfer rate. In this study, ferrocene as an electron mediator is covalently bound to a 2D-MOL (Fc-NH2-Hf-BTB-MOL) to accelerate electron transfer between the electrode surface and enzyme. Glucose oxidase (GOx) is immobilized on the electrode modified with Fc-NH2-Hf-BTB-MOL with the addition of chitosan and carboxylated carbon nanotubes. Electrochemical tests such as cyclic voltammetry are carried out on the glucose biosensor, which shows linear detection ranges of 5 ~ 400 μM and 3 ~ 9 mM, with a detection limit of 3.9 μM (S/N = 3). Therefore, this strategy of construction of an enzyme electrode based on 2D-MOLs with enhanced electron transfer results in a biosensor with excellent specificity and activity for practical glucose detection.