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<p>Rehabilitation for wound care involves a specialized program designed to promote wound healing. This often requires a multidisciplinary team and innovative therapies to enhance healing. However, the discovery of new therapeutics for wound care is limited by issues such as microbial resistance. Levofloxacin (LEV), an antibiotic used to treat bacterial and fungal infections, faces challenges due to its poor bioavailability and limited clinical usage. In this study, we developed a pH-responsive delivery system to effectively release LEV at targeted sites. We successfully encapsulated LEV within multifunctional ZIF-90 nanocrystals coated with a chitosan (CS) polymer. The designed CS@LEV-ZIF-90 NPs enable a pH-responsive antibiotic release mechanism that is particularly effective in acidic environments. This controlled drug release kinetics of ZIF-90 nanocrystals facilitates the rapid delivery of LEV within microbial cells. Our findings demonstrate that the combination of zinc and LEV exhibits a synergistic antibacterial effect, resulting in zones of inhibition of 26&#xa0;mm against <i>Escherichia coli</i> (<i>E. coli</i>) and 16&#xa0;mm against <i>Staphylococcus aureus</i> (<i>S. aureus</i>). Moreover, live/dead antibiofilm assays indicate that the CS@LEV-ZIF-90 NPs can eliminate approximately 80% of biofilm-forming infectious pathogens at the lowest tested dose. Additionally, antifungal tests reveal a significant reduction in colony growth against wound-infecting fungi, such as <i>Candida albicans</i> and <i>Aspergillus fumigatus</i>. Importantly, CS@LEV-ZIF-90 NPs exhibit a potential anti-inflammatory effect against LPS-stimulated RAW 264.7 cells, which enhances wound healing and alleviates the inflammation. The biocompatibility investigation confirms that the synthesized CS@LEV-ZIF-90 NPs are non-toxic for clinical applications. Overall, this research highlights the potential of these nanoparticles as an effective and innovative therapeutic option for nursing care of wound healing and rehabilitation in public health.</p>

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Fabrication of stimuli-responsive chitosan-coated levofloxacin-incorporated zeolite imidazole nanoparticles for nursing care of wound healing

  • 期刊论文

  • 发表日期：

    2026年1月8日

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  • Yakun Zhang,
  • Cuiping Zhang

摘要

Rehabilitation for wound care involves a specialized program designed to promote wound healing. This often requires a multidisciplinary team and innovative therapies to enhance healing. However, the discovery of new therapeutics for wound care is limited by issues such as microbial resistance. Levofloxacin (LEV), an antibiotic used to treat bacterial and fungal infections, faces challenges due to its poor bioavailability and limited clinical usage. In this study, we developed a pH-responsive delivery system to effectively release LEV at targeted sites. We successfully encapsulated LEV within multifunctional ZIF-90 nanocrystals coated with a chitosan (CS) polymer. The designed CS@LEV-ZIF-90 NPs enable a pH-responsive antibiotic release mechanism that is particularly effective in acidic environments. This controlled drug release kinetics of ZIF-90 nanocrystals facilitates the rapid delivery of LEV within microbial cells. Our findings demonstrate that the combination of zinc and LEV exhibits a synergistic antibacterial effect, resulting in zones of inhibition of 26 mm against Escherichia coli (E. coli) and 16 mm against Staphylococcus aureus (S. aureus). Moreover, live/dead antibiofilm assays indicate that the CS@LEV-ZIF-90 NPs can eliminate approximately 80% of biofilm-forming infectious pathogens at the lowest tested dose. Additionally, antifungal tests reveal a significant reduction in colony growth against wound-infecting fungi, such as Candida albicans and Aspergillus fumigatus. Importantly, CS@LEV-ZIF-90 NPs exhibit a potential anti-inflammatory effect against LPS-stimulated RAW 264.7 cells, which enhances wound healing and alleviates the inflammation. The biocompatibility investigation confirms that the synthesized CS@LEV-ZIF-90 NPs are non-toxic for clinical applications. Overall, this research highlights the potential of these nanoparticles as an effective and innovative therapeutic option for nursing care of wound healing and rehabilitation in public health.