Scholarlink

<p>Understanding how nanoplastics (NPLs) exposure affects vascular endothelium is essential for determining their potential cardiovascular risk. To this end, four different NPLs of similar nominal sizes (about 200&#xa0;nm), but different environmental relevance, have been used. They are: (i) spherical and monodisperse pristine polystyrene (PS), (ii) biodegradable polylactic acid (PLA), (iii) moderately irregular and polydisperse polytetrafluoroethylene (PTFE), and (iv) highly irregular and polydisperse polyethylene terephthalate (PET) derived from post-consumer bottles. To determine their hazardous risk, primary human umbilical vein endothelial cells (HUVECs) were used as a physiologically relevant model of the vascular endothelium. Results show that all NPLs were internalized by HUVECs, although uptake efficiency and intracellular distribution varied among polymers. None of the NPLs induced cytotoxicity or DNA damage at 25&#xa0;µg/mL for 24&#xa0;h. However, PTFE- and PET-NPLs elicited functional alterations consistent with endothelial dysfunction. PET-NPLs triggered IL-6 secretion and intracellular cholesterol accumulation, while both PTFE- and PET-NPLs significantly impaired cell migration, reducing wound closure. These findings reveal a clear gradient of biological impact, with irregular NPLs inducing stronger endothelial stress responses. By linking morphological realism to vascular inflammation, cholesterol dysregulation, and impaired migration, this study underscores the relevance of environmentally realistic NPLs into human health risk assessment frameworks.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Real-life nanoplastics induce endothelial dysfunction in primary human endothelial cells

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年5月5日

查看全文
  • Joan Martín-Pérez,
  • Michelle Morataya-Reyes,
  • Aliro Villacorta,
  • Claudia Anguita-Solé,
  • Juan Francisco Ferrer,
  • Irene Barguilla,
  • Mohamed Alaraby,
  • Ricard Marcos,
  • Alba Hernández,
  • Alba García-Rodríguez

摘要

Understanding how nanoplastics (NPLs) exposure affects vascular endothelium is essential for determining their potential cardiovascular risk. To this end, four different NPLs of similar nominal sizes (about 200 nm), but different environmental relevance, have been used. They are: (i) spherical and monodisperse pristine polystyrene (PS), (ii) biodegradable polylactic acid (PLA), (iii) moderately irregular and polydisperse polytetrafluoroethylene (PTFE), and (iv) highly irregular and polydisperse polyethylene terephthalate (PET) derived from post-consumer bottles. To determine their hazardous risk, primary human umbilical vein endothelial cells (HUVECs) were used as a physiologically relevant model of the vascular endothelium. Results show that all NPLs were internalized by HUVECs, although uptake efficiency and intracellular distribution varied among polymers. None of the NPLs induced cytotoxicity or DNA damage at 25 µg/mL for 24 h. However, PTFE- and PET-NPLs elicited functional alterations consistent with endothelial dysfunction. PET-NPLs triggered IL-6 secretion and intracellular cholesterol accumulation, while both PTFE- and PET-NPLs significantly impaired cell migration, reducing wound closure. These findings reveal a clear gradient of biological impact, with irregular NPLs inducing stronger endothelial stress responses. By linking morphological realism to vascular inflammation, cholesterol dysregulation, and impaired migration, this study underscores the relevance of environmentally realistic NPLs into human health risk assessment frameworks.