Scholarlink

<p>Immunocompromised patients, especially hematopoietic stem cell transplants (HSCT) / bone marrow transplant recipients, are at risk for colonization and infection with carbapenem-resistant <i>Klebsiella pneumoniae</i> (CR-KP) which is associated with increased bloodstream infections and mortality in this population. In this susceptible population, we investigated the molecular epidemiology of CR-KP. The strains isolated over a five-year period (2017–2021) were then characterized by PCR and sequencing targeting carbapenemase genes. ERIC-PCR was utilized to examine clonal relations, while multilocus sequence typing was carried out on representative isolates. Analysis of 142 CR-KP uncovered remarkable genetic heterogeneity with six predominant clusters making up for 83,8% of strains and one major cluster. Novel findings in <i>K. pneumoniae</i> included: undescribed MLST profile ST5187, emergence of <i>bla</i><sub><i>VIM−1</i></sub> in ST397, emergence of <i>bla</i><sub><i>OXA−48−like</i></sub> in ST219, co-carriage of either <i>bla</i><sub><i>NDM−1</i></sub> + <i>bla</i><sub><i>OXA−48−like</i></sub> in ST15 or <i>bla</i><sub><i>VIM-like</i></sub> + <i>bla</i><sub><i>NDM−1</i></sub> in ST405. CR-KP, brought to light new carbapenemase gene associations and undescribed MLST profiles. To prevent transmission of high-risk clones in immunocompromised populations, enhanced molecular surveillance and robust infection control are of high importance.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Emergence of gene associations in high-risk K. pneumoniae clones: blaNDM-1 + blaOXA-48-like in ST15 and blaVIM + blaNDM-1 in ST405

  • 期刊论文

  • 发表日期：

    2026年8月1日

查看全文
  • Ameni Ben Hamza,
  • Anis Raddaoui,
  • Siwar Frigui,
  • Yosra Chebbi,
  • Wafa Achour

摘要

Immunocompromised patients, especially hematopoietic stem cell transplants (HSCT) / bone marrow transplant recipients, are at risk for colonization and infection with carbapenem-resistant Klebsiella pneumoniae (CR-KP) which is associated with increased bloodstream infections and mortality in this population. In this susceptible population, we investigated the molecular epidemiology of CR-KP. The strains isolated over a five-year period (2017–2021) were then characterized by PCR and sequencing targeting carbapenemase genes. ERIC-PCR was utilized to examine clonal relations, while multilocus sequence typing was carried out on representative isolates. Analysis of 142 CR-KP uncovered remarkable genetic heterogeneity with six predominant clusters making up for 83,8% of strains and one major cluster. Novel findings in K. pneumoniae included: undescribed MLST profile ST5187, emergence of blaVIM−1 in ST397, emergence of blaOXA−48−like in ST219, co-carriage of either blaNDM−1 + blaOXA−48−like in ST15 or blaVIM-like + blaNDM−1 in ST405. CR-KP, brought to light new carbapenemase gene associations and undescribed MLST profiles. To prevent transmission of high-risk clones in immunocompromised populations, enhanced molecular surveillance and robust infection control are of high importance.