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<p>Strain HP20-50<sup>T</sup> was isolated from brine of a former salt mine in Solivar (Prešov, Slovakia). The cells are catalase- and oxidase-positive, aerobic Gram-negative short rods. Optimal growth occurred at temperature of 25&#xa0;°C, pH of 7.0, and a salinity range of 3.0% to 6.0% NaCl (<i>w/v</i>). Phylogenetic analysis based on the 16&#xa0;S rRNA gene sequences affiliated the strain with the genus <i>Idiomarina</i>, with the highest similarity to <i>Idiomarina ramblicola</i> R22<sup>T</sup> (99.6%), followed by <i>Idiomarina loihiensis</i> L2TR<sup>T</sup> (99.5%). Comparative whole-genome analyses of strain HP20-50<sup>T</sup> with its closest related type strains of the genus <i>Idiomarina</i> showed average nucleotide identity (ANI) values ranging from 65.0% to 82.8%, average amino acid identity (AAI) values from 67.1% to 91.2%, and digital DNA–DNA hybridization (dDDH) values from 18.2% to 23.2%. The major fatty acids were C<sub>11:0</sub> iso 3-OH (20.1%), C<sub>13:0</sub> iso 3-OH (15.5%), C<sub>11:0</sub> iso (10.0%), and C<sub>12:0</sub> 3-OH (9.0%), with 3-OH fatty acids predominance, a pattern never observed in <i>Idiomarina</i> species neither other halophilic bacteria. Strain HP20-50<sup>T</sup> has a genome DNA G + C content of 46.8% and a genome size of 2.8&#xa0;Mb. Based on the genomic analyses and the unique phenotypic properties of strain HP20-50ᵀ, we propose that the strain represents a novel species of the genus <i>Idiomarina</i>, for which the name <i>Idiomarina solivarensis</i> sp. nov. is proposed. The type strain is HP20-50<sup>T</sup> (= DSM 120110<sup>T</sup> = CCM 9475<sup>T</sup>).</p>

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Idiomarina solivarensis sp. nov., halophilic bacterium isolated from brine of the former subsurface salt mine in Solivar (Slovakia) showing a unique fatty acids profile

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  • 发表日期：

    2026年6月16日

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  • Soňa Brestovičová,
  • Lea Nosáľová,
  • Jana Kisková,
  • Lukáš Malčický,
  • Peter Pristaš

摘要

Strain HP20-50T was isolated from brine of a former salt mine in Solivar (Prešov, Slovakia). The cells are catalase- and oxidase-positive, aerobic Gram-negative short rods. Optimal growth occurred at temperature of 25 °C, pH of 7.0, and a salinity range of 3.0% to 6.0% NaCl (w/v). Phylogenetic analysis based on the 16 S rRNA gene sequences affiliated the strain with the genus Idiomarina, with the highest similarity to Idiomarina ramblicola R22T (99.6%), followed by Idiomarina loihiensis L2TRT (99.5%). Comparative whole-genome analyses of strain HP20-50T with its closest related type strains of the genus Idiomarina showed average nucleotide identity (ANI) values ranging from 65.0% to 82.8%, average amino acid identity (AAI) values from 67.1% to 91.2%, and digital DNA–DNA hybridization (dDDH) values from 18.2% to 23.2%. The major fatty acids were C11:0 iso 3-OH (20.1%), C13:0 iso 3-OH (15.5%), C11:0 iso (10.0%), and C12:0 3-OH (9.0%), with 3-OH fatty acids predominance, a pattern never observed in Idiomarina species neither other halophilic bacteria. Strain HP20-50T has a genome DNA G + C content of 46.8% and a genome size of 2.8 Mb. Based on the genomic analyses and the unique phenotypic properties of strain HP20-50ᵀ, we propose that the strain represents a novel species of the genus Idiomarina, for which the name Idiomarina solivarensis sp. nov. is proposed. The type strain is HP20-50T (= DSM 120110T = CCM 9475T).