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<p><i>Limosilactobacillus fermentum</i> 5.1.2, a tempeh-derived probiotic candidate, was investigated through an integrated genome-to-cell approach to evaluate its antioxidant capacity. Whole-genome analysis using GhostKOALA (KEGG) and antiSMASH 7.0 identified a terpene precursor biosynthetic gene cluster and key genes involved in terpenoid backbone (<i>idi</i>, <i>GGPS</i>) and terpenoid-quinone biosynthesis (<i>menA</i>, <i>menB</i>, <i>menC</i>, <i>menE</i>, <i>ubiE</i>). LC-QTOF-MS of the cell-free supernatant extract putatively identified terpenoid and terpenoid-quinone metabolites, including linalyl propionate, geranylbenzoquinone, geranylhydroquinone, theasapogenol A, and momordol. This is consistent with the predicted biosynthetic pathways. The cell-free supernatant exhibited strong DPPH radical-scavenging activity (84.85–88.99% inhibition), while the extract showed antioxidant activity against DPPH and ABTS+ radicals with IC<sub>50</sub> values of 660.5647 ± 35.67&#xa0;µg/mL and 333.886 ± 13.13&#xa0;µg/mL, respectively. Extract conferred cellular protection to <i>Schizosaccharomyces pombe</i> under H₂O₂-induced oxidative stress, comparable to calorie-restriction and ascorbic acid controls. These findings establish <i>L. fermentum</i> 5.1.2 as a multifunctional probiotic candidate with genomically supported antioxidant capacity.</p>

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Genetic potential of Limosilactobacillus fermentum 5.1.2 produces metabolite compounds with antioxidant activity

  • 期刊论文

  • 发表日期：

    2026年6月30日

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  • Vidia Nur Riska Parakkasi,
  • Radhwa Hayyu Aufa Haq,
  • Rika Indri Astuti,
  • Anja Meryandini

摘要

Limosilactobacillus fermentum 5.1.2, a tempeh-derived probiotic candidate, was investigated through an integrated genome-to-cell approach to evaluate its antioxidant capacity. Whole-genome analysis using GhostKOALA (KEGG) and antiSMASH 7.0 identified a terpene precursor biosynthetic gene cluster and key genes involved in terpenoid backbone (idi, GGPS) and terpenoid-quinone biosynthesis (menA, menB, menC, menE, ubiE). LC-QTOF-MS of the cell-free supernatant extract putatively identified terpenoid and terpenoid-quinone metabolites, including linalyl propionate, geranylbenzoquinone, geranylhydroquinone, theasapogenol A, and momordol. This is consistent with the predicted biosynthetic pathways. The cell-free supernatant exhibited strong DPPH radical-scavenging activity (84.85–88.99% inhibition), while the extract showed antioxidant activity against DPPH and ABTS+ radicals with IC50 values of 660.5647 ± 35.67 µg/mL and 333.886 ± 13.13 µg/mL, respectively. Extract conferred cellular protection to Schizosaccharomyces pombe under H₂O₂-induced oxidative stress, comparable to calorie-restriction and ascorbic acid controls. These findings establish L. fermentum 5.1.2 as a multifunctional probiotic candidate with genomically supported antioxidant capacity.