Scholarlink

<p>The global production of <i>Penaeus vannamei</i> shrimp is threatened by <i>Vibrio parahaemolyticus</i>, a pathogen causing severe disease outbreaks and economic losses, especially in Asia. This study developed and characterized stable sunflower oil-based nanoemulsions (NEs) and incorporated them into formulated feed for targeted antimicrobial control. Physicochemical analyses (particle size, zeta potential, viscosity, stability) confirmed uniform formulations. Antimicrobial efficacy was evaluated using MIC/MBC, adherence, biofilm inhibition, oxygen consumption rate (OCR), and virulence gene expression assays. NE-23 and NE-27 showed strong antibacterial activity (MIC/MBC: 1–256/2–170&#xa0;µg/mL), consistent with gentamicin. NE-22 exhibited the highest anti-adherence activity (70.62%), while NE-23 and NE-21 were most effective against pathogenic strains. Biofilm inhibition reached 72.17% (NE-22, MTCC-451) and 59–60% (NE-23, shrimp isolates), comparable to gentamicin. Dead cell assays revealed NE-24 induced up to 68.52% mortality, surpassing gentamicin in some strains. OCR analysis showed greater inhibition by NE-22 (0.31-fold reduction) than gentamicin (0.66-fold). Gene expression profiling demonstrated relative downregulation by NE-23. Finally, nanoemulsion-infused formulated feed conferred in vivo protection against <i>V. parahaemolyticus</i> without affecting shrimp growth or survival. Overall, NE-22 and NE-23 emerged as potent antibiotic alternatives, offering a sustainable strategy to mitigate <i>V. parahaemolyticus</i> infections in shrimp aquaculture.</p> Graphical abstract <p></p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Integrated nanoemulsion based functional feed against Vibrio parahaemolyticus pathogenesis in Penaeus vannamei: physicochemical characterization and in vivo efficacy

  • 期刊论文

  • 发表日期：

    2026年3月17日

查看全文
  • Jahangir Ahmed,
  • K. P. Kumaraguru Vasagam,
  • Karthikeyan Ramalingam

摘要

The global production of Penaeus vannamei shrimp is threatened by Vibrio parahaemolyticus, a pathogen causing severe disease outbreaks and economic losses, especially in Asia. This study developed and characterized stable sunflower oil-based nanoemulsions (NEs) and incorporated them into formulated feed for targeted antimicrobial control. Physicochemical analyses (particle size, zeta potential, viscosity, stability) confirmed uniform formulations. Antimicrobial efficacy was evaluated using MIC/MBC, adherence, biofilm inhibition, oxygen consumption rate (OCR), and virulence gene expression assays. NE-23 and NE-27 showed strong antibacterial activity (MIC/MBC: 1–256/2–170 µg/mL), consistent with gentamicin. NE-22 exhibited the highest anti-adherence activity (70.62%), while NE-23 and NE-21 were most effective against pathogenic strains. Biofilm inhibition reached 72.17% (NE-22, MTCC-451) and 59–60% (NE-23, shrimp isolates), comparable to gentamicin. Dead cell assays revealed NE-24 induced up to 68.52% mortality, surpassing gentamicin in some strains. OCR analysis showed greater inhibition by NE-22 (0.31-fold reduction) than gentamicin (0.66-fold). Gene expression profiling demonstrated relative downregulation by NE-23. Finally, nanoemulsion-infused formulated feed conferred in vivo protection against V. parahaemolyticus without affecting shrimp growth or survival. Overall, NE-22 and NE-23 emerged as potent antibiotic alternatives, offering a sustainable strategy to mitigate V. parahaemolyticus infections in shrimp aquaculture.

Graphical abstract