Scholarlink

<p>The development of resistance to antibiotics and bacteriophages highlights the necessity of alternative therapy for <i>Staphylococcus aureus.</i> This study aimed to explore the utility of temperate phages as a biocontrol agent against multidrug-resistant (MDR) <i>S. aureus</i>. Four <i>S. aureus</i> temperate phages were successfully isolated, and their biological features, genomic properties, and antibacterial effect against <i>S. aureus</i> and biofilm were characterised. Phage SapYZUs891 exhibited relatively high titre (1.7 × 10<sup>10</sup> PFU/mL), short latent period (5 min), large burst size (554 PFU/cell), strong pH (4–10) and thermal stability (25–70&#xa0;°C), and a broad lytic spectrum (47.3%, 43/91; <i>p</i> ≤ 0.05). Comparative genomic analysis suggested that SapYZUs891 lacks antibiotic resistance or virulence genes, but contains unique gene content, including genes encoding DNA polymerase, DNA packaging protein, holin, and tail fibre protein. Notably, SapYZUs891 significantly inhibited biofilm formation (28.3%—70.6%, <i>p</i> &lt; 0.01), scavenged mature <i>S. aureus</i> biofilms (36.9%—61.5%, <i>p</i> &lt; 0.001) and reduced the counts of <i>S. aureus</i> (1.14 – 1.40 Lg CFU/mL, <i>p</i> ≤ 0.01) in milk at 4°C. Furthermore, SapYZUs891 effectively reduced the minimum inhibitory concentrations of MDR <i>S. aureus</i> against antibiotics by 2 – 64 folds (<i>p</i> ≤ 0.05). Therefore, the temperate phage SapYZUs891 with attractive biological properties, genetic features and antibacterial potential is an effective biocontrol agent against MDR <i>S. aureus</i>.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Effect of temperate phage SapYZUs891 as a biocontrol agent on the Chinese foodborne multidrug-resistant Staphylococcus aureus isolates

  • 期刊论文

  • 发表日期：

    2026年2月16日

查看全文
  • Xiaoxing Fan,
  • Zhilan Yao,
  • Yuhong Wu,
  • Wenjuan Li,
  • Yeling Han,
  • Xuan Li,
  • Aiping Deng,
  • Guoqiang Zhu,
  • Zhenquan Yang,
  • Wenyuan Zhou

摘要

The development of resistance to antibiotics and bacteriophages highlights the necessity of alternative therapy for Staphylococcus aureus. This study aimed to explore the utility of temperate phages as a biocontrol agent against multidrug-resistant (MDR) S. aureus. Four S. aureus temperate phages were successfully isolated, and their biological features, genomic properties, and antibacterial effect against S. aureus and biofilm were characterised. Phage SapYZUs891 exhibited relatively high titre (1.7 × 1010 PFU/mL), short latent period (5 min), large burst size (554 PFU/cell), strong pH (4–10) and thermal stability (25–70 °C), and a broad lytic spectrum (47.3%, 43/91; p ≤ 0.05). Comparative genomic analysis suggested that SapYZUs891 lacks antibiotic resistance or virulence genes, but contains unique gene content, including genes encoding DNA polymerase, DNA packaging protein, holin, and tail fibre protein. Notably, SapYZUs891 significantly inhibited biofilm formation (28.3%—70.6%, p < 0.01), scavenged mature S. aureus biofilms (36.9%—61.5%, p < 0.001) and reduced the counts of S. aureus (1.14 – 1.40 Lg CFU/mL, p ≤ 0.01) in milk at 4°C. Furthermore, SapYZUs891 effectively reduced the minimum inhibitory concentrations of MDR S. aureus against antibiotics by 2 – 64 folds (p ≤ 0.05). Therefore, the temperate phage SapYZUs891 with attractive biological properties, genetic features and antibacterial potential is an effective biocontrol agent against MDR S. aureus.