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<p>Probiotics<?tk 2?> are live microorganisms that confer health benefits when administered in adequate amounts, enhance immune function, promote gut health, and are increasingly recognized as effective alternatives to antibiotics for improving animal health and productivity. This study investigated the probiotic potential of lactic acid bacteria (LAB) from <i>Mystus cavasius</i> through morphological, physiological, and molecular characterization for aquaculture feed applications. Out of twenty-two presumptive LAB isolates, eleven were sequenced after biochemical screening, and molecular analysis confirmed eight as LAB (<i>Weisella confusa</i> ML1, ML2, ML3, ML6, ML7; <i>Enterococcus faecalis</i> ML8, ML9; <i>Lactococcus garvieae</i> ML10). The study also found a potential non-LAB probiotic bacterium <i>Bacillus subtilis</i> ML4. Moreover, the study identified <i>Paraburkholderia phytofirmans</i>, a potential plant probiotic bacterium, for the first time in fish. Among the identified LAB isolates, <i>L. garvieae</i> ML10 was excluded from probiotic potential assessments because of its reported disease-causing potential and associated health risks. All the tested LAB isolates were found to tolerate acidic pH (2–5) and bile salts (2.5–7.5%), indicating their probiotic potential for aquaculture applications. Additionally, the isolates showed no hemolytic activity and demonstrated strong probiotic potential, characterized by high cell surface hydrophobicity, ranging from 41.98 ± 16.68% to 62.19 ± 4.09% in xylene and 51.58 ± 1.67% to 64.83 ± 4.91% in toluene, robust autoaggregation (55.51 ± 3.63% to 64.33 ± 2.87%) and substantial coaggregation with <i>Staphylococcus haemolyticus</i> (46.27 ± 2.96% to 53.54 ± 0.72%) and <i>Bacillus cereus</i> (34.34 ± 3.16% to 51.76 ± 4.08%). Furthermore, they exhibited potent in vitro inhibitory effects against the fish pathogens <i>Aeromonas veronii</i> and <i>L. garvieae</i> and revealed susceptibility to several commonly used antibiotics. Therefore, the results revealed that the selected LAB isolates might be ideal probiotic candidates to be used in sustainable aquaculture practices.<?tk 0?></p>

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Isolation and molecular characterization of gut-derived lactic acid bacteria from the gangetic mystus, Mystus Cavasius with promising probiotic attributes

  • 期刊论文

  • 发表日期：

    2026年2月2日

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  • Chandrika Mondal,
  • Md. Sakhawat Hossain,
  • Rafiatul Jannat Rifa,
  • Md Imtiaz Ahamed,
  • Tanjila Akhter Tanni,
  • Zakir Hossain,
  • Tanvir Rahman,
  • Mahbubul Pratik Siddique,
  • Mariom

摘要

Probiotics are live microorganisms that confer health benefits when administered in adequate amounts, enhance immune function, promote gut health, and are increasingly recognized as effective alternatives to antibiotics for improving animal health and productivity. This study investigated the probiotic potential of lactic acid bacteria (LAB) from Mystus cavasius through morphological, physiological, and molecular characterization for aquaculture feed applications. Out of twenty-two presumptive LAB isolates, eleven were sequenced after biochemical screening, and molecular analysis confirmed eight as LAB (Weisella confusa ML1, ML2, ML3, ML6, ML7; Enterococcus faecalis ML8, ML9; Lactococcus garvieae ML10). The study also found a potential non-LAB probiotic bacterium Bacillus subtilis ML4. Moreover, the study identified Paraburkholderia phytofirmans, a potential plant probiotic bacterium, for the first time in fish. Among the identified LAB isolates, L. garvieae ML10 was excluded from probiotic potential assessments because of its reported disease-causing potential and associated health risks. All the tested LAB isolates were found to tolerate acidic pH (2–5) and bile salts (2.5–7.5%), indicating their probiotic potential for aquaculture applications. Additionally, the isolates showed no hemolytic activity and demonstrated strong probiotic potential, characterized by high cell surface hydrophobicity, ranging from 41.98 ± 16.68% to 62.19 ± 4.09% in xylene and 51.58 ± 1.67% to 64.83 ± 4.91% in toluene, robust autoaggregation (55.51 ± 3.63% to 64.33 ± 2.87%) and substantial coaggregation with Staphylococcus haemolyticus (46.27 ± 2.96% to 53.54 ± 0.72%) and Bacillus cereus (34.34 ± 3.16% to 51.76 ± 4.08%). Furthermore, they exhibited potent in vitro inhibitory effects against the fish pathogens Aeromonas veronii and L. garvieae and revealed susceptibility to several commonly used antibiotics. Therefore, the results revealed that the selected LAB isolates might be ideal probiotic candidates to be used in sustainable aquaculture practices.