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<p>This research aimed for the safety and probiotic potential assessment of <i>Pediococcus pentosaceus</i> L1, isolated from pickled radish, through its whole genome sequencing analysis and in vitro evaluation. The genome size of <i>Ped. pentosaceus</i> L1 was 1,751,467&#xa0;bp long with an average guanine-cytosine (GC) ratio of 37.41% and contained 1739 genes in the coding region. The functional annotation of the L1 strain demonstrated that it exhibited excellent probiotic potential and health-promoting properties, with numerous genes linked to the carbohydrate metabolism system, stress resistance, and environmental adaptation. The analysis of virulence factor and antimicrobial resistance indicated the absence of any virulence-related or antibiotic resistance genes in <i>Ped. pentosaceus</i> L1, adding value to its safety profile. In vitro experiments showed that <i>Ped. pentosaceus</i> L1 presented high cell surface hydrophobicity, strong antioxidant activity, no hemolytic activity, and did not synthesize biogenic amines including cadaverine, putrescine, or agmatine. Additionally, its auto- and co-aggregation survival rates indicated its probiotic potential. <i>Ped. pentosaceus</i> L1 also exhibited exceptional exopolysaccharide (EPS) production potential, achieving an enhanced EPS yield in a glucose-supplemented medium. Together, all these characteristics make <i>Ped. pentosaceus</i> L1 an optimistically safe choice for use as a potential probiotic.</p>

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Whole genome sequencing to assess the safety and probiotic potential of Pediococcus pentosaceus L1 isolated from pickled radish

  • 期刊论文

  • 发表日期：

    2025年11月5日

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  • Ghulam Mustafa,
  • Chen Liu,
  • Dongmei Wang,
  • Jijiang Dong,
  • Jinping Zhou,
  • Laifeng Chen,
  • Yini Ma,
  • Zhenhui Cao,
  • Le Xu,
  • Qiuye Lin

摘要

This research aimed for the safety and probiotic potential assessment of Pediococcus pentosaceus L1, isolated from pickled radish, through its whole genome sequencing analysis and in vitro evaluation. The genome size of Ped. pentosaceus L1 was 1,751,467 bp long with an average guanine-cytosine (GC) ratio of 37.41% and contained 1739 genes in the coding region. The functional annotation of the L1 strain demonstrated that it exhibited excellent probiotic potential and health-promoting properties, with numerous genes linked to the carbohydrate metabolism system, stress resistance, and environmental adaptation. The analysis of virulence factor and antimicrobial resistance indicated the absence of any virulence-related or antibiotic resistance genes in Ped. pentosaceus L1, adding value to its safety profile. In vitro experiments showed that Ped. pentosaceus L1 presented high cell surface hydrophobicity, strong antioxidant activity, no hemolytic activity, and did not synthesize biogenic amines including cadaverine, putrescine, or agmatine. Additionally, its auto- and co-aggregation survival rates indicated its probiotic potential. Ped. pentosaceus L1 also exhibited exceptional exopolysaccharide (EPS) production potential, achieving an enhanced EPS yield in a glucose-supplemented medium. Together, all these characteristics make Ped. pentosaceus L1 an optimistically safe choice for use as a potential probiotic.